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Product Info Summary
| SKU: | A06852 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-AMOTL2 Antibody Picoband®
SKU/Catalog Number
A06852
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMOTL2 Antibody Picoband® catalog # A06852. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMOTL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06852)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human AMOTL2 recombinant protein (Position: A401-Q480).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06852 is reactive to AMOTL2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
85.764kDa
Background of AMOTL2
Angiomotin-like protein 2 is a protein that in humans is encoded by the AMOTL2 gene. Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). It mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06852 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, rat brain tissue, rat lung tissue, mouse lung tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of AMOTL2 using anti-AMOTL2 antibody (A06852).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMOTL2 antigen affinity purified polyclonal antibody (Catalog # A06852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMOTL2 at approximately 95KD. The expected band size for AMOTL2 is at 86KD.
Click image to see more details
Figure 2. Western blot analysis of AMOTL2 using anti-AMOTL2 antibody (A06852).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMOTL2 antigen affinity purified polyclonal antibody (Catalog # A06852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMOTL2 at approximately 95KD. The expected band size for AMOTL2 is at 86KD.
Protein Target Info & Infographic
Gene/Protein Information For AMOTL2 (Source: Uniprot.org, NCBI)
Gene Name
AMOTL2
Full Name
Angiomotin-like protein 2
Weight
85.764kDa
Superfamily
angiomotin family
Alternative Names
Angiomotin-like protein 2; Leman coiled-coil protein; LCCP; AMOTL2; KIAA0989 AMOTL2 LCCP angiomotin like 2 angiomotin-like protein 2|Leman coiled-coil protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMOTL2, check out the AMOTL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMOTL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMOTL2 Antibody Picoband® (A06852)
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Customer Q&As
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4 Customer Q&As for Anti-AMOTL2 Antibody Picoband®
Question
Is a blocking peptide available for product anti-AMOTL2 antibody (A06852)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do provide the blocking peptide for product anti-AMOTL2 antibody (A06852). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-06
Question
Will anti-AMOTL2 antibody A06852 work for WB with placenta teratocarcinoma?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
According to the expression profile of placenta teratocarcinoma, AMOTL2 is highly expressed in placenta teratocarcinoma. So, it is likely that anti-AMOTL2 antibody A06852 will work for WB with placenta teratocarcinoma.
Boster Scientific Support
Answered: 2020-03-18
Question
We are currently using anti-AMOTL2 antibody A06852 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
L. Li
Verified customer
Asked: 2019-07-19
Answer
The anti-AMOTL2 antibody (A06852) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19
Question
Does A06852 anti-AMOTL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
You can see on the product datasheet, A06852 anti-AMOTL2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-24
