Click image to see more details
Product Info Summary
| SKU: | M08382-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody
View all alpha Tubulin 4a Antibodies
SKU/Catalog Number
M08382-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody catalog # M08382-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08382-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CG-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human alpha Tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08382-1 is reactive to TUBA1B in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
50152 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08382-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human A549 whole cell, human K562 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
IHC: human bladder tissue
ICC/IF: 293 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Alpha Tubulin using anti-Alpha Tubulin antibody (M08382-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Tubulin antigen affinity purified monoclonal antibody (Catalog # M08382-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Tubulin at approximately 55 kDa. The expected band size for Alpha Tubulin is at 55 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody.
Click image to see more details
Immunofluorescent analysis of 293 cells, using alpha Tubulin Antibody .
Protein Target Info & Infographic
Gene/Protein Information For TUBA1B (Source: Uniprot.org, NCBI)
Gene Name
TUBA1B
Full Name
Tubulin alpha-4A chain
Weight
50152 MW
Superfamily
tubulin family
Alternative Names
Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain;TUBA1B; TUBA4A ALS22, H2-ALPHA, TUBA1 tubulin alpha 4a tubulin alpha-4A chain|tubulin H2-alpha|tubulin alpha-1 chain|tubulin, alpha 1 (testis specific)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBA1B, check out the TUBA1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBA1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody (M08382-1)
Hello CJ!
M08382-1 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CpG Oligodeoxynucleotides Downregulate Placental Adiponectin and Increase Embryo Loss in Non%u2010Obese Diabetic Mice
Validation of Bmi1 as a therapeutic target of hepatocellular carcinoma in mice
Stem cell autocrine CXCL12/CXCR4 stimulates invasion and metastasis of esophageal cancer
Prognostic relevance of melanoma antigen D1 expression in colorectal carcinoma
Baclofen protects primary rat retinal ganglion cells from chemical hypoxia-induced apoptosis through the Akt and PERK pathways
NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder
Prohibitin protects against oxidative stress-induced cell injury in cultured neonatal cardiomyocyte
Endogenous Nodal promotes melanoma undergoing epithelial-mesenchymal transition via Snail and Slug in vitro and in vivo.
Overexpression of p53 activated by small activating RNA suppresses the growth of human prostate cancer cells
The Signaling Mechanism of TGF-?1 Induced Bovine Mammary Epithelial Cell Apoptosis
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-alpha Tubulin TUBA1B Rabbit Monoclonal Antibody
Question
My question regarding product M08382-1, anti-alpha Tubulin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08382-1 anti-alpha Tubulin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-04
Question
Does anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 work on primate IHC with keratinocyte?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Our lab technicians have not validated anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate keratinocyte in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-05
Question
Is a blocking peptide available for product anti-alpha Tubulin Rabbit Monoclonal antibody (M08382-1)?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
We do provide the blocking peptide for product anti-alpha Tubulin Rabbit Monoclonal antibody (M08382-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-01
Question
I was wanting to use your anti-alpha Tubulin Rabbit Monoclonal antibody for IHC for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
It shows on the product datasheet, M08382-1 anti-alpha Tubulin Rabbit Monoclonal antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-25
Question
I am looking for to test anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 on mouse placenta for research purposes, then I may be interested in using anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Carter
Verified customer
Asked: 2016-11-03
Answer
The products we sell, including anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-03
Question
I see that the anti-alpha Tubulin Rabbit Monoclonal antibody M08382-1 works with IHC, what is the protocol used to produce the result images on the product page?
B. Taylor
Verified customer
Asked: 2013-07-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-07-26
