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Product Info Summary
| SKU: | MA1107 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A)
View all alpha Tubulin Antibodies
SKU/Catalog Number
MA1107
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) catalog # MA1107. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1107)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
DM1A
Isotype
Mouse IgG1
Immunogen
Microtubules from chicken embryo brain.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1107 is reactive to TUBA1A in Chicken, Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
50136 MW
Background of alpha Tubulin
Alpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1107 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, chicken, By Heat
Western blot, 1μg/ml, Human, mouse, rat, chicken
Positive Control
WB: human SH-SY5Y whole cell, human Jurkat whole cell, human A549 whole cell, rat brain tissue, mouse brain tissue, mouse thymus tissue
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of alpha-Tubulin using anti-alpha-Tubulin antibody (MA1107).
alpha-Tubulin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-alpha-Tubulin Antibody (MA1107) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 2. Western blot analysis of alpha-Tubulin using anti-alpha-Tubulin antibody (MA1107).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-alpha-Tubulin antigen affinity purified monoclonal antibody (Catalog # MA1107) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for alpha-Tubulin at approximately 55 kDa. The expected band size for alpha-Tubulin is at 50 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TUBA1A (Source: Uniprot.org, NCBI)
Gene Name
TUBA1A
Full Name
Tubulin alpha-1A chain
Weight
50136 MW
Superfamily
tubulin family
Alternative Names
Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1A;TUBA3; TUBA1A B-ALPHA-1, LIS3, TUBA3 tubulin alpha 1a tubulin alpha-1A chain|hum-a-tub1|hum-a-tub2|tubulin B-alpha-1|tubulin alpha-3 chain|tubulin, alpha, brain-specific
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBA1A, check out the TUBA1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) (MA1107)
Hello CJ!
MA1107 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody‐antigen interaction and increased specificity
Yu-Yuan Chen,Yin-Peng Bai,Bin Li,Xiao-Bo Zhao,Cheng-Jie Yang,Ying-Qian Liu,Jian-Mei Gao,Jun Guo,Chun Li,Jing-Wen Peng,Zhong-Min Zhao,Zhi-Jun Zhang,Chuan-Rui Xu,Design and Synthesis of Novel 20(S)-α-aminophosphonate Derivatives of Camptothecin as Potent Antitumor Agents,Bioorganic Chemistry,2021,105065,ISSN 0045-2068, https://doi.org/10.1016/j.bioorg.2021.105065.
Species: Human,Mouse
MA1107 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:1:1000
Danyang Chong,Zhong Chen,Shan Guan,Tongyu Zhang,Na Xu,Yue Zhao,Chaojun Li,Geranylgeranyl pyrophosphate-mediated protein geranylgeranylation regulates endothelial cell proliferation and apoptosis during vasculogenesis in mouse embryo,Journal of Genetics and Genomics,2021,,ISSN 1673-8527,https://doi.org/10.1016/j.jgg. 2021.03.009.
Species: Mouse
MA1107 usage in article: APP:WB, SAMPLE: HUVECS, DILUTION:1:1000
Chen Li,LinLin Chen,YuanYuan Wang et al. Protein Nanoparticle Osmotic Pressure Modifies Nonselective Permeability of the Blood Brain Barrier by Increasing Membrane Fluidity, 04 November 2020, PREPRINT (Version 1) available at Research Square [+https://doi
Species: Human,Rat
MA1107 usage in article: APP:WB, SAMPLE:HBMEC CELLS, DILUTION:NA
Yuan Y,Li B,Kuang Y,Ni S,Zhuge A,Yang J,Lv L,Gu S,Yan R,Li Y,Wang K,Yang L,Zhu X,Wu J,Bian X,Li L. The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells. Cancer Cell Int.2020 Jun 3;20:212.doi:10. 1186/s12935-020-01305-9
Species: Human,Mouse
Ni S,Kuang Y,Yuan Y,Yu B. Mitochondrion-mediated iron accumulation promotes carcinogenesis and Warburg effect through reactive oxygen species in osteosarcoma. Cancer Cell Int.2020 Aug 18;20:399. doi:10.1186/s12935-020-01494-3.PMID:32831652;PMCID:PMC743701
Species: Human,Mouse
MA1107 usage in article: APP:WB, SAMPLE:SAOS-2 CELL AND U2OS CELL, DILUTION:1:1000
Zhou H, Wu G, Ma X, Xiao J, Yu G, Yang C, Xu N, Zhang B, Zhou J, Ye Z, Wang Z. J Exp Clin Cancer Res. 2018 Apr 27;37(1):89. doi: 10.1186/s13046-018-0764-9. Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse ...
Zhang X, Wu N. Drug Des Devel Ther. 2018 Feb 23;12:373-381. doi: 10.2147/DDDT.S147547. eCollection 2018. Fasudil inhibits proliferation and migration of Hep-2 laryngeal carcinoma cells
Schwartz, K., & Bochkariov, D. (2017). Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity. Electrophoresis. Advance online publication. doi: 10.1002/elp...
Chen Xh, Bao Yy, Zhou Sh, Wang Qy, Wei Y, Fan J. Mol Med Rep. 2013 Dec;8(6):1695-700. Doi: 10.3892/Mmr.2013.1740. Epub 2013 Oct 18. Glucose Transporter-1 Expression In Cd133+ Laryngeal Carcinoma Hep-2 Cells.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A)
Question
Does anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 work on monkey WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Our lab technicians have not tested anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-05
Question
Our lab want to know about to test anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 on mouse fetal brain for research purposes, then I may be interested in using anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
The products we sell, including anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-21
Question
Will anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 work for IHC with fetal brain?
M. Moore
Verified customer
Asked: 2017-08-22
Answer
According to the expression profile of fetal brain, TUBA1A is highly expressed in fetal brain. So, it is likely that anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 will work for IHC with fetal brain.
Boster Scientific Support
Answered: 2017-08-22
Question
We are currently using anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
The anti-Alpha-Tubulin antibody (Monoclonal, DM1A) (MA1107) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-23
