Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A)

alpha Tubulin antibody

Boster Bio Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) catalog # MA1107. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1107
Size: 100 μg/vial
Reactive Species: Chicken, Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

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Product Name

Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A)

View all alpha Tubulin Antibodies

SKU/Catalog Number

MA1107

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) catalog # MA1107. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1107)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DM1A

Isotype

Mouse IgG1

Immunogen

Microtubules from chicken embryo brain.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1107 is reactive to TUBA1A in Chicken, Human, Mouse, Rat

Applications

MA1107 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

50.136kDa

Background of alpha Tubulin

Alpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, chicken, By Heat
Western blot, 1μg/ml, Human, mouse, rat, chicken

Validation Images & Assay Conditions

Gene/Protein Information For TUBA1A (Source: Uniprot.org, NCBI)

Gene Name

TUBA1A

Full Name

Tubulin alpha-1A chain

Weight

50.136kDa

Superfamily

tubulin family

Alternative Names

alpha tubulin loading control; alpha tubulin monoclonal; alpha Tubulin; Alpha-Tubulin 3; B-ALPHA-1; DM1A tubulin; dm1a monoclonal; DM1a tubulin; DM1A; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TUBA1 monoclonal; TUBA1A; TUBA3; tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-Alpha-1; tubulin monoclonal; tubulin, alpha 1a; tubulin, alpha 3; tubulin, alpha, brain-specific TUBA1A B-ALPHA-1, LIS3, TUBA3 tubulin alpha 1a tubulin alpha-1A chain|hum-a-tub1|hum-a-tub2|tubulin B-alpha-1|tubulin alpha-3 chain|tubulin, alpha, brain-specific

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TUBA1A, check out the TUBA1A Infographic

TUBA1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TUBA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

MA1107 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody‐antigen interaction and increased specificity

Yu-Yuan Chen,Yin-Peng Bai,Bin Li,Xiao-Bo Zhao,Cheng-Jie Yang,Ying-Qian Liu,Jian-Mei Gao,Jun Guo,Chun Li,Jing-Wen Peng,Zhong-Min Zhao,Zhi-Jun Zhang,Chuan-Rui Xu,Design and Synthesis of Novel 20(S)-α-aminophosphonate Derivatives of Camptothecin as Potent Antitumor Agents,Bioorganic Chemistry,2021,105065,ISSN 0045-2068, https://doi.org/10.1016/j.bioorg.2021.105065.
Species: Human,Mouse
MA1107 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:1:1000

Danyang Chong,Zhong Chen,Shan Guan,Tongyu Zhang,Na Xu,Yue Zhao,Chaojun Li,Geranylgeranyl pyrophosphate-mediated protein geranylgeranylation regulates endothelial cell proliferation and apoptosis during vasculogenesis in mouse embryo,Journal of Genetics and Genomics,2021,,ISSN 1673-8527,https://doi.org/10.1016/j.jgg. 2021.03.009.
Species: Mouse
MA1107 usage in article: APP:WB, SAMPLE: HUVECS, DILUTION:1:1000

Chen Li,LinLin Chen,YuanYuan Wang et al. Protein Nanoparticle Osmotic Pressure Modifies Nonselective Permeability of the Blood Brain Barrier by Increasing Membrane Fluidity, 04 November 2020, PREPRINT (Version 1) available at Research Square [+https://doi
Species: Human,Rat
MA1107 usage in article: APP:WB, SAMPLE:HBMEC CELLS, DILUTION:NA

Yuan Y,Li B,Kuang Y,Ni S,Zhuge A,Yang J,Lv L,Gu S,Yan R,Li Y,Wang K,Yang L,Zhu X,Wu J,Bian X,Li L. The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells. Cancer Cell Int.2020 Jun 3;20:212.doi:10. 1186/s12935-020-01305-9
Species: Human,Mouse

Ni S,Kuang Y,Yuan Y,Yu B. Mitochondrion-mediated iron accumulation promotes carcinogenesis and Warburg effect through reactive oxygen species in osteosarcoma. Cancer Cell Int.2020 Aug 18;20:399. doi:10.1186/s12935-020-01494-3.PMID:32831652;PMCID:PMC743701
Species: Human,Mouse
MA1107 usage in article: APP:WB, SAMPLE:SAOS-2 CELL AND U2OS CELL, DILUTION:1:1000

Zhou H, Wu G, Ma X, Xiao J, Yu G, Yang C, Xu N, Zhang B, Zhou J, Ye Z, Wang Z. J Exp Clin Cancer Res. 2018 Apr 27;37(1):89. doi: 10.1186/s13046-018-0764-9. Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse ...

Zhang X, Wu N. Drug Des Devel Ther. 2018 Feb 23;12:373-381. doi: 10.2147/DDDT.S147547. eCollection 2018. Fasudil inhibits proliferation and migration of Hep-2 laryngeal carcinoma cells

Schwartz, K., & Bochkariov, D. (2017). Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity. Electrophoresis. Advance online publication. doi: 10.1002/elp...

Chen Xh, Bao Yy, Zhou Sh, Wang Qy, Wei Y, Fan J. Mol Med Rep. 2013 Dec;8(6):1695-700. Doi: 10.3892/Mmr.2013.1740. Epub 2013 Oct 18. Glucose Transporter-1 Expression In Cd133+ Laryngeal Carcinoma Hep-2 Cells.

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4 Customer Q&As for Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A)

Question

Does anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 work on monkey WB with brain?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

Our lab technicians have not tested anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-05

Question

Our lab want to know about to test anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 on mouse fetal brain for research purposes, then I may be interested in using anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-03-21

Answer

The products we sell, including anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-21

Question

Will anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 work for IHC with fetal brain?

M. Moore

Verified customer

Asked: 2017-08-22

Answer

According to the expression profile of fetal brain, TUBA1A is highly expressed in fetal brain. So, it is likely that anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 will work for IHC with fetal brain.

Boster Scientific Support

Answered: 2017-08-22

Question

We are currently using anti-Alpha-Tubulin antibody (Monoclonal, DM1A) MA1107 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-23

Answer

The anti-Alpha-Tubulin antibody (Monoclonal, DM1A) (MA1107) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-23

Order DetailsPrice
MA1107

100μg

$370
MA1107-10ug

10μg sample (liquid)

$99
MA1107-Biotin

100 μg Biotin conjugated

$570
MA1107-Cy3

100 μg Cy3 conjugated

$570
MA1107-Dylight488

100 μg Dylight488 conjugated

$570
MA1107-Dylight550

100 μg Dylight550 conjugated

$570
MA1107-Dylight594

100 μg Dylight594 conjugated

$570
MA1107-FITC

100 μg FITC conjugated

$570
MA1107-HRP

100 μg HRP conjugated

$570
MA1107-APC

100 μg APC conjugated

$670
MA1107-PE

100 μg PE conjugated

$670
MA1107-carrier-free

Carrier Free

$370

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MA1107
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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