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Product Info Summary
| SKU: | A03989 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-alpha-Tubulin TUBA1A Antibody
View all alpha Tubulin Antibodies
SKU/Catalog Number
A03989
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-alpha-Tubulin TUBA1A Antibody (Catalog # A03989). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
alpha-Tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-alpha-Tubulin TUBA1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03989)
Host
Rabbit
Contents
alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
alpha-Tubulin antibody is human, mouse and rat reactive.
Reactive Species
A03989 is reactive to TUBA1A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
50136 MW
Background of alpha Tubulin
alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03989 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of alpha-Tubulin in 293
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Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 2.5 μg/ml.
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Immunofluorescence of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 20 μg/mL.
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Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TUBA1A (Source: Uniprot.org, NCBI)
Gene Name
TUBA1A
Full Name
Tubulin alpha-1A chain
Weight
50136 MW
Superfamily
tubulin family
Alternative Names
Tubulin alpha-1A, TUBA1A, TUBA3, LIS3 TUBA1A B-ALPHA-1, LIS3, TUBA3 tubulin alpha 1a tubulin alpha-1A chain|hum-a-tub1|hum-a-tub2|tubulin B-alpha-1|tubulin alpha-3 chain|tubulin, alpha, brain-specific
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBA1A, check out the TUBA1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha-Tubulin TUBA1A Antibody (A03989)
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No publications found for A03989
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