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Product Info Summary
| SKU: | A00522 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband®
View all alpha-Fetoprotein/AFP Antibodies
SKU/Catalog Number
A00522
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband® catalog # A00522. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00522)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Alpha 1 Fetoprotein recombinant protein (Position: K19-L254).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00522 is reactive to Afp in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.678kDa
Background of alpha-Fetoprotein/AFP
RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3, is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00522 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse HEPA1-6 cell, rat RH35 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Alpha 1 Fetoprotein using anti-Alpha 1 Fetoprotein antibody (A00522).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse HEPA1-6 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Fetoprotein antigen affinity purified polyclonal antibody (Catalog # A00522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Fetoprotein at approximately 69KD. The expected band size for Alpha 1 Fetoprotein is at 69KD.
Click image to see more details
Figure 2. Western blot analysis of Alpha 1 Fetoprotein using anti-Alpha 1 Fetoprotein antibody (A00522).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 Fetoprotein antigen affinity purified polyclonal antibody (Catalog # A00522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 Fetoprotein at approximately 69KD. The expected band size for Alpha 1 Fetoprotein is at 69KD.
Protein Target Info & Infographic
Gene/Protein Information For Afp (Source: Uniprot.org, NCBI)
Gene Name
Afp
Full Name
Alpha-fetoprotein
Weight
68.678kDa
Superfamily
ALB/AFP/VDB family
Alternative Names
Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp AFP AFPD, FETA, HPAFP alpha fetoprotein alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Afp, check out the Afp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Afp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband® (A00522)
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Customer Q&As
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16 Customer Q&As for Anti-Alpha 1 Fetoprotein/Afp Antibody Picoband®
Question
Do you have a BSA free version of anti-Alpha 1 Fetoprotein/Afp antibody A00522 available?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Alpha 1 Fetoprotein/Afp antibody A00522 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-15
Question
Our team were happy with the WB result of your anti-Alpha 1 Fetoprotein/Afp antibody. However we have been able to see positive staining in embryo secreted. using this antibody. Is that expected? Could you tell me where is AFP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
According to literature, embryo does express AFP. Generally AFP expresses in secreted. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-01
Question
Is a blocking peptide available for product anti-Alpha 1 Fetoprotein/Afp antibody (A00522)?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We do provide the blocking peptide for product anti-Alpha 1 Fetoprotein/Afp antibody (A00522). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo using anti-Alpha 1 Fetoprotein/Afp antibody A00522. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-22
Question
Is this A00522 anti-Alpha 1 Fetoprotein/Afp antibody reactive to the isotypes of AFP?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The immunogen of A00522 anti-Alpha 1 Fetoprotein/Afp antibody is E. coli-derived mouse Alpha 1 Fetoprotein recombinant protein (Position: K19-L254). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-28
Question
I am looking for using your anti-Alpha 1 Fetoprotein/Afp antibody for ovulation from ovarian follicle studies. Has this antibody been tested with western blotting on rat rh35 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
Thank you for your inquiry. This A00522 anti-Alpha 1 Fetoprotein/Afp antibody is validated on rat rh35 whole cell lysates. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-14
Question
My question regards to test anti-Alpha 1 Fetoprotein/Afp antibody A00522 on rat embryo for research purposes, then I may be interested in using anti-Alpha 1 Fetoprotein/Afp antibody A00522 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
The products we sell, including anti-Alpha 1 Fetoprotein/Afp antibody A00522, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-12
Question
Would anti-Alpha 1 Fetoprotein/Afp antibody A00522 work for WB with embryo?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
According to the expression profile of embryo, AFP is highly expressed in embryo. So, it is likely that anti-Alpha 1 Fetoprotein/Afp antibody A00522 will work for WB with embryo.
Boster Scientific Support
Answered: 2019-07-11
Question
We are currently using anti-Alpha 1 Fetoprotein/Afp antibody A00522 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-Alpha 1 Fetoprotein/Afp antibody (A00522) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
We have tried in the past anti-Alpha 1 Fetoprotein/Afp antibody for ELISA on lung a few months ago. I am using rat, and We want to use the antibody for WB next. We are interested in examining lung as well as embryo in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
I looked at the website and datasheets of our anti-Alpha 1 Fetoprotein/Afp antibody and it appears that A00522 has been tested on rat in both ELISA and WB. Thus A00522 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-10-16
Question
We have seen staining in rat embryo. Any tips? Is anti-Alpha 1 Fetoprotein/Afp antibody supposed to stain embryo positively?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
Based on literature embryo does express AFP. Based on Uniprot.org, AFP is expressed in embryo, heart, lung, brain cajal-retzius cell, among other tissues. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-23
Question
Can you help my question with product A00522, anti-Alpha 1 Fetoprotein/Afp antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Collins
Verified customer
Asked: 2017-05-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00522 anti-Alpha 1 Fetoprotein/Afp antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-17
Question
I was wanting to use your anti-Alpha 1 Fetoprotein/Afp antibody for WB for rat embryo on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat embryo identification?
B. Jackson
Verified customer
Asked: 2014-10-14
Answer
You can see on the product datasheet, A00522 anti-Alpha 1 Fetoprotein/Afp antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryo in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-14
Question
Does A00522 anti-Alpha 1 Fetoprotein/Afp antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Johnson
Verified customer
Asked: 2014-01-21
Answer
You can see on the product datasheet, A00522 anti-Alpha 1 Fetoprotein/Afp antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-01-21
Question
I see that the anti-Alpha 1 Fetoprotein/Afp antibody A00522 works with WB, what is the protocol used to produce the result images on the product page?
N. Parker
Verified customer
Asked: 2013-12-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-12-23
Question
Please see the WB image, lot number and protocol we used for embryo using anti-Alpha 1 Fetoprotein/Afp antibody A00522. Please let me know if you require anything else.
S. Banerjee
Verified customer
Asked: 2013-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-17
