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Product Info Summary
| SKU: | A06325 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-ALKBH2 Antibody
SKU/Catalog Number
A06325
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ALKBH2 Antibody (Catalog # A06325). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ALKBH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ALKBH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06325)
Host
Rabbit
Contents
ALKBH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ALKBH2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ALKBH2. The immunogen is located within amino acids 180 - 230 of ALKBH2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ZNF521 antibody is human specific.
Reactive Species
A06325 is reactive to ALKBH2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
29322 MW
Background of ABH2
The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA; ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06325 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ALKBH2 antibody can be used for detection of ALKBH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ALKBH2 in human kidney tissue lysate with ALKBH2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of ALKBH2 in human kidney tissue with ALKBH2 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ALKBH2 (Source: Uniprot.org, NCBI)
Gene Name
ALKBH2
Full Name
DNA oxidative demethylase ALKBH2
Weight
29322 MW
Superfamily
alkB family
Alternative Names
ABH2, ABH2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Alkylated DNA repair protein alkB homolog 2 ALKBH2 ABH2 alkB homolog 2, alpha-ketoglutarate dependent dioxygenase DNA oxidative demethylase ALKBH2|2OG-Fe(II) oxy DC1|alkB, alkylation repair homolog 2|alkylated DNA repair protein alkB homolog 2|alpha-ketoglutarate-dependent dioxygenase alkB homolog 2|oxy DC1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALKBH2, check out the ALKBH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALKBH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALKBH2 Antibody (A06325)
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