Anti-AGR3 Purified Monoclonal Antibody

Product Name

Anti-AGR3 Purified Monoclonal Antibody

View all AG-3/AGR3 Antibodies

SKU/Catalog Number

M05950

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-AGR3 Purified Monoclonal Antibody (Catalog# M05950). Tested in WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-AGR3 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05950)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

AGR3.1

Isotype

Mouse IgG1

Immunogen

Purified human AGR3 protein. The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Cross-reactivity with CLIC5b was not determined.

Reactive Species

M05950 is reactive to AGR3 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

19171 MW

Background of AG-3/AGR3

AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05950 is guaranteed for IHC-P, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 1 μg/ml; positive tissue: breast cancer.
Immunohistochemistry (frozen sections): 1 μg/ml; positive tissue: breast cancer.
Western blotting: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.

Validation Images & Assay Conditions

Gene/Protein Information For AGR3 (Source: Uniprot.org, NCBI)

Alternative Names

AG3, HAG3 AGR3 AG-3, AG3, BCMP11, HAG3, PDIA18, hAG-3 anterior gradient 3, protein disulphide isomerase family member anterior gradient protein 3|anterior gradient homolog 3|anterior gradient protein 3 homolog|breast cancer membrane protein 11|protein disulfide isomerase family A, member 18

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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