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Product Info Summary
| SKU: | M05441 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ADX Rabbit Monoclonal Antibody
SKU/Catalog Number
M05441
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ADX Rabbit Monoclonal Antibody catalog # M05441. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADX Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05441)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28F80
Isotype
IgG
Immunogen
A synthesized peptide derived from human ADX
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05441 is reactive to FDX1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
13 kDa
Calculated molecular weight
50327 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05441 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human A549 whole cell, human CACO-2 whole cell, human SiHa whole cell, human 293T whole cell, rat PC-12 whole cell, rat C6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FDX1 using anti-FDX1 antibody (M05441).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FDX1 antigen affinity purified monoclonal antibody (Catalog # M05441) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FDX1 at approximately 13 kDa. The expected band size for FDX1 is at 19 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For FDX1 (Source: Uniprot.org, NCBI)
Gene Name
FDX1
Full Name
Adrenodoxin, mitochondrial
Weight
50327 MW
Superfamily
adrenodoxin/putidaredoxin family
Alternative Names
Tubulin beta-1 chain;TUBB1; FDX1 ADX, FDX, LOH11CR1D ferredoxin 1 adrenodoxin, mitochondrial|adrenal ferredoxin|hepatoredoxin|mitochondrial adrenodoxin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FDX1, check out the FDX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FDX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADX Rabbit Monoclonal Antibody (M05441)
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