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Product Info Summary
| SKU: | A05578-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Adgrg1 Antibody Picoband®
SKU/Catalog Number
A05578-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Adgrg1 Antibody Picoband® catalog # A05578-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Adgrg1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05578-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Adgrg1 recombinant protein (Position: E53-S554).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05578-2 is reactive to Adgrg1 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
48608 MW
Background of GPR56
Enables several functions, including collagen binding activity; extracellular matrix binding activity; and heparin binding activity. Involved in several processes, including negative regulation of neuron migration; positive regulation of Rho protein signal transduction; and seminiferous tubule development. Acts upstream of or within cerebral cortex regionalization; hematopoietic stem cell homeostasis; and positive regulation of neural precursor cell proliferation. Located in glial limiting end-foot. Is expressed in several structures, including alimentary system; ganglia; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in bilateral frontoparietal polymicrogyria and bilateral perisylvian polymicrogyria. Orthologous to human ADGRG1 (adhesion G protein-coupled receptor G1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05578-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Mouse
ELISA, 0.1-0.5 µg/ml, Mouse
Positive Control
WB: rat brain tissue, rat heart tissue, rat C6 whole cell, mouse brain tissue, mosue heart tissue, mouse Neuro-2a whole cell, mouse C2C12 whole cell
IHC: mouse brain tissue, rat brain tissue
FCM: RAW2647 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Adgrg1 using anti-Adgrg1 antibody (A05578-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mosue heart tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates,
Lane 7: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adgrg1 antigen affinity purified polyclonal antibody (Catalog # A05578-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adgrg1 at approximately 78 kDa. The expected band size for Adgrg1 is at 78 kDa.
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Figure 2. IHC analysis of Adgrg1 using anti-Adgrg1 antibody (A05578-2).
Adgrg1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Adgrg1 Antibody (A05578-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of Adgrg1 using anti-Adgrg1 antibody (A05578-2).
Adgrg1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Adgrg1 Antibody (A05578-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of RAW264.7 cells using anti-Adgrg1 antibody (A05578-2).
Overlay histogram showing RAW264.7 cells stained with A05578-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Adgrg1 Antibody (A05578-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Adgrg1 (Source: Uniprot.org, NCBI)
Gene Name
Adgrg1
Full Name
Adhesion G-protein coupled receptor G1
Weight
48608 MW
Superfamily
G-protein coupled receptor 2 family
Alternative Names
Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB ADGRG1 BFPP, BPPR, GPR56, TM7LN4, TM7XN1 adhesion G protein-coupled receptor G1 adhesion G-protein coupled receptor G1|7-transmembrane protein with no EGF-like N-terminal domains-1|G protein-coupled receptor 56|testicular tissue protein Li 77
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Adgrg1, check out the Adgrg1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Adgrg1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adgrg1 Antibody Picoband® (A05578-2)
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