Product Info Summary
SKU: | M03673 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-ACTN2 Rabbit Monoclonal Antibody
View all Alpha Actinin 2 Antibodies
SKU/Catalog Number
M03673
BM4907 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ACTN2 Rabbit Monoclonal Antibody catalog # M03673. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTN2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03673)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOCB-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ACTN2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03673 is reactive to ACTN2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
104 kDa
Calculated molecular weight
103854 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03673 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: HepG2 cell, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ACTN2 expression in HepG2 cell lysate.
Click image to see more details
Figure 1. Western blot analysis of ACTN2 using anti-ACTN2 antibody (M03673).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN2 antigen affinity purified monoclonal antibody (Catalog # M03673) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTN2 at approximately 104 kDa. The expected band size for ACTN2 is at 104 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ACTN2 (Source: Uniprot.org, NCBI)
Gene Name
ACTN2
Full Name
Alpha-actinin-2
Weight
103854 MW
Superfamily
alpha-actinin family
Alternative Names
Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein;ACTN2; ACTN2 CMD1AA, CMH23, MPD6, MYOCOZ actinin alpha 2 alpha-actinin-2|F-actin cross-linking protein|alpha-actinin skeletal muscle|truncated actinin alpha 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTN2, check out the ACTN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACTN2 Rabbit Monoclonal Antibody (M03673)
Hello CJ!
M03673 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Similar to Spironolactone, Oxymatrine Is Protective in Aldosterone-Induced Cardiomyocyte Injury via Inhibition of Calpain and Apoptosis-Inducing Factor Signaling
Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes
Li H, Tang M, Liang H, Li Y, Wang J, Song Y, Zheng Y, Xi J, Zhang J, Hescheler J, Zhu M. Cell Physiol Biochem. 2013;32(1):53-63. Doi: 10.1159/000350124. Epub 2013 Jul 4. Coculture Of Embryonic Ventricular Myocytes And Mouse Embryonic Stem Cell Enh...
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Customer Q&As
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3 Customer Q&As for Anti-ACTN2 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-ACTN2 Rabbit Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
You can see on the product datasheet, M03673 anti-ACTN2 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
Our lab want to know about to test anti-ACTN2 Rabbit Monoclonal antibody M03673 on human brain for research purposes, then I may be interested in using anti-ACTN2 Rabbit Monoclonal antibody M03673 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The products we sell, including anti-ACTN2 Rabbit Monoclonal antibody M03673, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-28
Question
Is this M03673 anti-ACTN2 Rabbit Monoclonal antibody reactive to the isotypes of ACTN2?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
The immunogen of M03673 anti-ACTN2 Rabbit Monoclonal antibody is A synthesized peptide derived from human ACTN2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-08