Product Info Summary
SKU: | A01072 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody
View all alpha-Smooth Muscle Actin Antibodies
SKU/Catalog Number
A01072
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody catalog # A01072. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01072)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
4A4
Isotype
IgG
Immunogen
Synthesized peptide of human ACTA2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01072 is reactive to ACTA2 in Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
42009 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01072 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
FC, 1:200-1:400
ELISA, 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis using ACTA2 Monoclonal antibody against HeLa (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
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Immunohistochemistry (IHC) analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) with DAB staining using ACTA2 Monoclonal antibody.
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Flow cytometric (FCM) analysis of HeLa cells using ACTA2 Monoclonal antibody (green) and negative control (purple).
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Immunofluorescence (IF) analysis of HepG2 cells using ACTA2 Monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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ELISA analysis of ACTA2 antibody.
Protein Target Info & Infographic
Gene/Protein Information For ACTA2 (Source: Uniprot.org, NCBI)
Gene Name
ACTA2
Full Name
Actin, aortic smooth muscle
Weight
42009 MW
Superfamily
actin family
Alternative Names
Actin, aortic smooth muscle;Alpha-actin-2;Cell growth-inhibiting gene 46 protein;ACTA2;ACTSA, ACTVS;GIG46; ACTA2 ACTSA actin alpha 2, smooth muscle actin, aortic smooth muscle|actin, alpha 2, smooth muscle, aorta|alpha-cardiac actin|cell growth-inhibiting gene 46 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTA2, check out the ACTA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody (A01072)
Hello CJ!
A01072 has been cited in 78 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivo
Mast cell degranulator compound 48–80 promotes atherosclerotic plaque in apolipoprotein E knockout mice with perivascular common carotid collar placement
Endothelial Cell Apoptosis is Responsible for the Formation of Coronary Thrombotic Atherosclerotic Plaques
Naringin and Naringenin Relax Rat Tracheal Smooth by Regulating BKCa Activation
The deregulation of STIM1 and store operative calcium entry impaired aortic smooth muscle cells contractility in aortic medial degeneration
Heme oxygenase-1 gene modified human placental mesenchymal stem cells promote placental angiogenesis and spiral artery remodeling by improving the balance of angiogenic factors in vitro
MeCP2 triggers diabetic cardiomyopathy and cardiac fibroblast proliferation by inhibiting RASSF1A
The GABAB Receptor Inhibits Activation of Hepatic Stellate Cells
Fang L,Wang KK,Zhang PF,Li T,Xiao ZL,Yang M,Yu ZX.Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF-BB.J Cell Mol Med.2020 Jan;24(2) :1917-1933.doi:10.1111/jcmm.14888.Epub 2020 Jan 1.PMI
Species: Rat
A01072 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
Shen J,Wei W,Wang X,Yang J,Lu L,Lv X,Xue X.Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation.Evid Based Complement Alternat Med.2020 Aug 22;2020:72
Species: Rat
A01072 usage in article: APP:IHC, SAMPLE:VSMCS, DILUTION:1:1000
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