Product Info Summary
SKU: | A08344 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-ABCB8 Antibody
SKU/Catalog Number
A08344
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ABCB8 Antibody (Catalog # A08344). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-ABCB8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08344)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
ABCB8 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a internal portion of human ABCB8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08344 is reactive to ABCB8 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
54341 MW
Background of ABCB8
This antibody is suitable for Cancer Research and drug resistance. It has been found that ABC transporters are involved in mulitdrug resistance through their ability to control the presence of drugs in cells. Multidrug resistance is currently being looked at in particular reference to cancer that is resistant to treatment. ABCB8 seems to have particularly high resistance rates in renal cancer and cancer of the central nervous system. Another significant aspect of ABCB8 is that it is a mitochondrial transporter. There are very few ABC transporters localized to the mitochondria which implies information about them could be extremely important. ABCB8 may also be localized to other parts of the cell which are yet to be determined. Anti-ABCB8 antibody is ideal for researchers interested in Cancer Research and drug resistance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08344 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:50,000
WB: 1:250-1:500
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABCB8 using anti-ABCB8 antibody (A08344).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB8 antigen affinity purified polyclonal antibody (Catalog # A08344) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ABCB8.
Protein Target Info & Infographic
Gene/Protein Information For ABCB8 (Source: Uniprot.org, NCBI)
Gene Name
ABCB8
Full Name
Mitochondrial potassium channel ATP-binding subunit
Weight
54341 MW
Superfamily
ABC transporter superfamily
Alternative Names
ABCB8, ATP-binding cassette sub-family B member 8 mitochondrial, Mitochondrial ATP-binding cassette 1, M-ABC1, ABCB-8, ABCB ABCB8 EST328128, M-ABC1, MABC1, MITOSUR ATP binding cassette subfamily B member 8 mitochondrial potassium channel ATP-binding subunit|ATP-binding cassette sub-family B member 8, mitochondrial|ATP-binding cassette, sub-family B (MDR/TAP), member 8|mitochondrial ABC protein|mitochondrial ATP-binding cassette 1|mitochondrial sulfonylurea-receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCB8, check out the ABCB8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCB8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCB8 Antibody (A08344)
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No publications found for A08344
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Customer Q&As
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6 Customer Q&As for Anti-ABCB8 Antibody
Question
Here is the WB image, lot number and protocol we used for kidney using anti-ABCB8 antibody A08344. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-ABCB8 antibody A08344 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-ABCB8 antibody (A08344) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
Will A08344 anti-ABCB8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
It shows on the product datasheet, A08344 anti-ABCB8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-20
Question
Will anti-ABCB8 antibody A08344 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
According to the expression profile of kidney, ABCB8 is highly expressed in kidney. So, it is likely that anti-ABCB8 antibody A08344 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-05-15
Question
Is a blocking peptide available for product anti-ABCB8 antibody (A08344)?
T. Edwards
Verified customer
Asked: 2016-01-21
Answer
We do provide the blocking peptide for product anti-ABCB8 antibody (A08344). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-01-21
Question
Will anti-ABCB8 antibody A08344 work on feline WB with left adrenal gland?
A. Edwards
Verified customer
Asked: 2014-08-07
Answer
Our lab technicians have not validated anti-ABCB8 antibody A08344 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ABCB8 antibody A08344 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline left adrenal gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-07