Product Info Summary
SKU: | A12404 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CAPN11/Calpain 11 Antibody
View all Calpain 11 Antibodies
SKU/Catalog Number
A12404
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CAPN11/Calpain 11 Antibody (Catalog # A12404). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAPN11/Calpain 11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12404)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CAPN11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12404 is reactive to CAPN11 in Human
Reconstitution
Calculated molecular weight
84423 MW
Background of Calpain 11
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Ota T., Nat. Genet. 36:40-45(2004).
Mungall A.J., Nature 425:805-811(2003).
Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12404 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, Jurkat cells and HuvEc cells, using CAPN11 antibody A12404.
Protein Target Info & Infographic
Gene/Protein Information For CAPN11 (Source: Uniprot.org, NCBI)
Gene Name
CAPN11
Full Name
Calpain-11
Weight
84423 MW
Superfamily
peptidase C2 family
Alternative Names
Calpain-11;3.4.22.-;Calcium-activated neutral proteinase 11;CANP 11;CAPN11; CAPN11 calpain11 calpain 11 calpain-11|CANP 11|calcium-activated neutral proteinase 11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAPN11, check out the CAPN11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAPN11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CAPN11/Calpain 11 Antibody (A12404)
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No publications found for A12404
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CAPN11/Calpain 11 Antibody
Question
I have attached the WB image, lot number and protocol we used for testis using anti-CAPN11/Calpain 11 antibody A12404. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-05
Question
I was wanting to use your anti-CAPN11/Calpain 11 antibody for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
As indicated on the product datasheet, A12404 anti-CAPN11/Calpain 11 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-21
Question
Would anti-CAPN11/Calpain 11 antibody A12404 work on horse WB with testis?
T. Williams
Verified customer
Asked: 2019-07-16
Answer
Our lab technicians have not tested anti-CAPN11/Calpain 11 antibody A12404 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CAPN11/Calpain 11 antibody A12404 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-16
Question
Does anti-CAPN11/Calpain 11 antibody A12404 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
According to the expression profile of testis, CAPN11 is highly expressed in testis. So, it is likely that anti-CAPN11/Calpain 11 antibody A12404 will work for WB with testis.
Boster Scientific Support
Answered: 2019-06-17
Question
My question regarding product A12404, anti-CAPN11/Calpain 11 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Zhang
Verified customer
Asked: 2018-09-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12404 anti-CAPN11/Calpain 11 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-04
Question
We are interested in to test anti-CAPN11/Calpain 11 antibody A12404 on human testis for research purposes, then I may be interested in using anti-CAPN11/Calpain 11 antibody A12404 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
The products we sell, including anti-CAPN11/Calpain 11 antibody A12404, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-20
Question
We are currently using anti-CAPN11/Calpain 11 antibody A12404 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
O. Parker
Verified customer
Asked: 2017-05-15
Answer
The anti-CAPN11/Calpain 11 antibody (A12404) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-15