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Product Info Summary
| SKU: | PA1373 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-5HT2A Receptor/HTR2A Antibody Picoband®
SKU/Catalog Number
PA1373
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-5HT2A Receptor/HTR2A Antibody catalog # PA1373. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-5HT2A Receptor/HTR2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1373)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2A Receptor, different from the mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1373 is reactive to HTR2A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
52603 MW
Background of 5-HT2A
The mammalian HTR2A (5-HT2A receptor) is a subtype of the 5-HT2 receptor that belongs to the serotonin receptor family and is a G protein-coupled receptor (GPCR). This is the main excitatory receptor subtype among the GPCRs for serotonin (5-HT), although 5-HT2A may also have an inhibitory effect on certain areas such as the visual cortex and the orbit frontal cortex. This receptor was given importance first as the target of psychedelic drugs like LSD. Later it came back to prominence because it was also found to be mediating, at least partly, the action of many antipsychotic drugs, especially the atypical ones.5-HT2A also happens to be a necessary receptor for the spread of the human polyoma virus called JC virus. Sparkes et al. (1991) concluded that the gene is located on 13q14-q21 in man and on chromosome 14 in the mouse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1373 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Rat Brain Tissue, Mouse Brain Tissue, Mouse Brain Tissue, U87 Cell, SMMC Cell, HT1080 Cell, COLO320 Cell
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
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Anti-5HT2A Receptor antibody, PA1373, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
Lane 5: U87 Cell Lysate
Lane 6: SMMC Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 8: COLO320 Cell Lysate
Click image to see more details
Anti-5HT2A Receptor antibody, PA1373, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For HTR2A (Source: Uniprot.org, NCBI)
Gene Name
HTR2A
Full Name
5-hydroxytryptamine receptor 2A
Weight
52603 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
5-hydroxytryptamine receptor 2A;5-HT-2;5-HT-2A;Serotonin receptor 2A;HTR2A;HTR2; HTR2A 5-HT2A, HTR2 5-hydroxytryptamine receptor 2A 5-hydroxytryptamine receptor 2A|5-HT2 receptor|5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled|serotonin 5-HT-2A receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTR2A, check out the HTR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-5HT2A Receptor/HTR2A Antibody Picoband® (PA1373)
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No publications found for PA1373
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Customer Reviews
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1 Reviews For Anti-5HT2A Receptor/HTR2A Antibody Picoband®
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It works far better compared to other vendors--Saikat Chakraborty, PhD, Feinberg School of Medicine, Northwestern University
Excellent
Source: Customer Feedback Submission
| Applications | Western Blot |
|---|---|
| Sample | Mouse brain lysate |
| Detection | Licor Imaging and ImageJ |
"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."
Saikat Chakraborty
Verified customer
Submitted 2017-11-29
Customer Q&As
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4 Customer Q&As for Anti-5HT2A Receptor/HTR2A Antibody Picoband®
Question
I see that the anti-5HT2A Receptor/HTR2A antibody PA1373 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-07
Question
I have attached the WB image, lot number and protocol we used for lung using anti-5HT2A Receptor/HTR2A antibody PA1373. Please let me know if you require anything else.
G. Miller
Verified customer
Asked: 2020-04-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-28
Question
Will anti-5HT2A Receptor/HTR2A antibody PA1373 work on feline WB with dorsolateral prefrontal cortex?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Our lab technicians have not validated anti-5HT2A Receptor/HTR2A antibody PA1373 on feline. You can run a BLAST between feline and the immunogen sequence of anti-5HT2A Receptor/HTR2A antibody PA1373 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline dorsolateral prefrontal cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
I have a question about product PA1373, anti-5HT2A Receptor/HTR2A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1373 anti-5HT2A Receptor/HTR2A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
