Product Info Summary
SKU: | M01687 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody
View all 14-3-3 epsilon Antibodies
SKU/Catalog Number
M01687
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody catalog # M01687. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01687)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HHD-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 14-3-3 epsilon
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01687 is reactive to YWHAE in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
29174 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01687 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
![m01687 wb m01687 wb](https://www.bosterbio.com/media/catalog/product/m/0/m01687-wb.jpg)
Click image to see more details
Western blot analysis of 14-3-3 epsilon expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For YWHAE (Source: Uniprot.org, NCBI)
Gene Name
YWHAE
Full Name
14-3-3 protein epsilon
Weight
29174 MW
Superfamily
14-3-3 family
Alternative Names
14-3-3 protein epsilon;14-3-3E;YWHAE; YWHAE 14-3-3E, HEL2, KCIP-1, MDCR, MDS tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon 14-3-3 protein epsilon|14-3-3 epsilon|epididymis luminal protein 2|mitochondrial import stimulation factor L subunit|protein kinase C inhibitor protein-1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide|tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YWHAE, check out the YWHAE Infographic
![YWHAE infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YWHAE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody (M01687)
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No publications found for M01687
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Customer Q&As
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2 Customer Q&As for Anti-14-3-3 epsilon YWHAE Rabbit Monoclonal Antibody
Question
Is this M01687 anti-14-3-3 epsilon Rabbit Monoclonal antibody reactive to the isotypes of YWHAE?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
The immunogen of M01687 anti-14-3-3 epsilon Rabbit Monoclonal antibody is A synthesized peptide derived from human 14-3-3 epsilon . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-23
Question
We are currently using anti-14-3-3 epsilon Rabbit Monoclonal antibody M01687 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
K. Baker
Verified customer
Asked: 2014-08-01
Answer
The anti-14-3-3 epsilon Rabbit Monoclonal antibody (M01687) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-01