Abl (Phospho-Tyr412) Colorimetric Cell-Based ELISA

ABL2 ELISA kit, phospho-specific, cell-based

The Abl (Phospho-Tyr412) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Abl (Phospho-Tyr412) protein expression profile in cells. The kit can be used for measuring the relative amounts of Abl (Phospho-Tyr412) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Abl (Phospho-Tyr412).

Product Info Summary

SKU: EKC2643
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

Abl (Phospho-Tyr412) Colorimetric Cell-Based ELISA

See all ABL2 products

SKU/Catalog Number

EKC2643

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The Abl (Phospho-Tyr412) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Abl (Phospho-Tyr412) protein expression profile in cells. The kit can be used for measuring the relative amounts of Abl (Phospho-Tyr412) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Abl (Phospho-Tyr412).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Abl (Phospho-Tyr412) Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2643)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2643 is reactive to ABL2 in Human, Mouse samples

Application Guarantee

EKC2643 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Abl Phospho-Tyr412 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-Abl Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For ABL2 (Source: Uniprot.Org, NCBI)

Gene Name

ABL2

Full Name

Tyrosine-protein kinase ABL2

Weight

128343 MW

Superfamily

protein kinase superfamily

Alternative Names

Tyrosine-protein kinase ABL2;2.7.10.2;Abelson murine leukemia viral oncogene homolog 2;Abelson tyrosine-protein kinase 2;Abelson-related gene protein;Tyrosine-protein kinase ARG;ABL2;ABLL, ARG; ABL2 ABLL, ARG ABL proto-oncogene 2, non-receptor tyrosine kinase tyrosine-protein kinase ABL2|Abelson tyrosine-protein kinase 2|abelson-related gene protein|c-abl oncogene 2, non-receptor tyrosine kinase|tyrosine-protein kinase ARG|v-abl Abelson murine leukemia viral oncogene homolog 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ABL2, check out the ABL2 Infographic

ABL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ABL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EKC2643

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Abl (Phospho-Tyr412) Colorimetric Cell-Based ELISA?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Abl (Phospho-Tyr412) Colorimetric Cell-Based ELISA

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Abl (Phospho-Tyr412) Colorimetric Cell-Based ELISA

$540
Backordered.

Lead time for this item is typically 5-7 days

Get A Quote
In stock
Order Product
EKC2643
$540.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.