Product Info Summary
SKU: | LS080818 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of zinc finger protein 436 (ZNF436), transcript variant 1 |
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Product info
Product Name
ZNF436 (NM_001077195) Human Over-expression Lysate
SKU/Catalog Number
LS080818
Size
100 µg
Description
Transient overexpression lysate of zinc finger protein 436 (ZNF436), transcript variant 1
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
ZNF436 (NM_001077195) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080818)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls080818 znf436 human over expression lysates nm 001077195 wb testing 1 ls080818 znf436 human over expression lysates nm 001077195 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls080818-znf436-human-over-expression-lysates-nm-001077195-wb-testing-1.jpg)
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ZNF436 Human Over-expression Lysates NM_001077195.
Western blot validation of overexpression lysate (LS080818) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZNF436 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
![ls080818 znf436 human over expression lysates nm 030634 wb testing 2 ls080818 znf436 human over expression lysates nm 030634 wb testing 2](https://www.bosterbio.com/media/catalog/product/l/s/ls080818-znf436-human-over-expression-lysates-nm-030634-wb-testing-2.jpg)
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ZNF436 Human Over-expression Lysates NM_030634.
Western blot validation of overexpression lysate (LS080818) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZNF436 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ZNF436 (Source: Uniprot.org, NCBI)
Gene Name
ZNF436
Full Name
Zinc finger protein 436
Weight
54.277kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
DKFZp686K08243; KRAB zinc finger protein HFB101; ZF2; zinc finger protein 274FLJ37843; Zinc finger protein HFB101; Zinc finger protein with KRAB and SCAN domains 19; Zinc finger protein zfp2; ZKSCAN19HFB101 ZNF436 ZNF, Zfp46 zinc finger protein 436 zinc finger protein 436|DNA-binding protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ZNF436, check out the ZNF436 Infographic
![ZNF436 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF436: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ZNF436 (NM_001077195) Human Over-expression Lysate (LS080818)
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