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Product Info Summary
| SKU: | LS023090 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of zinc finger protein 423 (ZNF423) |
Product info
Product Name
ZNF423 (NM_015069) Human Over-expression Lysate
SKU/Catalog Number
LS023090
Size
100 µg
Description
Transient overexpression lysate of zinc finger protein 423 (ZNF423)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
ZNF423 (NM_015069) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023090)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ZNF423 Human Over-expression Lysates NM_015069.
Western blot validation of overexpression lysate (LS023090) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZNF423 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ZNF423 (Source: Uniprot.org, NCBI)
Gene Name
ZNF423
Full Name
Zinc finger protein 423
Weight
144.605kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
FLJ20086; KIAA1584; MGC21637; MGC61687; SUHW4; Suppressor of hairy wing homolog 4; zinc finger protein 280D; Zinc finger protein 634; ZNF634suppressor of hairy wing homolog 4 (Drosophila) ZNF423 Ebfaz, JBTS19, NPHP14, OAZ, Roaz, ZFP423, Zfp104, hOAZ zinc finger protein 423 zinc finger protein 423|OLF-1/EBF associated zinc finger|Smad- and Olf-interacting zinc finger protein|early B-cell factor associated zinc finger protein|olf1/EBF-associated zinc finger protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ZNF423, check out the ZNF423 Infographic
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In this infographic, you will see the following information for ZNF423: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ZNF423 (NM_015069) Human Over-expression Lysate (LS023090)
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