Product Info Summary
SKU: | LS079726 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of WD repeat domain 59 (WDR59) |
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Product info
Product Name
WDR59 (NM_030581) Human Over-expression Lysate
SKU/Catalog Number
LS079726
Size
100 µg
Description
Transient overexpression lysate of WD repeat domain 59 (WDR59)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
WDR59 (NM_030581) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079726)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls079726 wdr59 human over expression lysates nm 030581 wb testing 1 ls079726 wdr59 human over expression lysates nm 030581 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls079726-wdr59-human-over-expression-lysates-nm-030581-wb-testing-1.jpg)
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WDR59 Human Over-expression Lysates NM_030581.
Western blot validation of overexpression lysate (LS079726) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with WDR59 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for WDR59 (Source: Uniprot.org, NCBI)
Gene Name
WDR59
Full Name
GATOR complex protein WDR59
Weight
109.793kDa
Superfamily
WD repeat WDR59 family
Alternative Names
FLJ12270; KIAA1923; MGC11230; WD repeat domain 59; WD repeat-containing protein 59 WDR59 CDW12, FP977, p90-120 WD repeat domain 59 GATOR complex protein WDR59|CDw12 antigen|WD repeat-containing protein 59
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on WDR59, check out the WDR59 Infographic
![WDR59 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WDR59: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For WDR59 (NM_030581) Human Over-expression Lysate (LS079726)
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