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Product Info Summary
| SKU: | EKC1834 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
VPS72 Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1834
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The VPS72 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor VPS72 protein expression profile in cells. The kit can be used for measuring the relative amounts of VPS72 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on VPS72.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
VPS72 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1834)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1834 is reactive to VPS72 in Human, Mouse samples
Application Guarantee
EKC1834 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-VPS72 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from RAW264.7/NIH-3T3 cells
Protein Target Info & Infographic
Gene/Protein Information For VPS72 (Source: Uniprot.Org, NCBI)
Gene Name
VPS72
Full Name
Vacuolar protein sorting-associated protein 72 homolog
Weight
40594 MW
Superfamily
VPS72/YL1 family
Alternative Names
Vacuolar protein sorting-associated protein 72 homolog;Protein YL-1;Transcription factor-like 1;VPS72;TCFL1, YL1; VPS72 CFL1, Swc2, TCFL1, YL-1, YL1 vacuolar protein sorting 72 homolog vacuolar protein sorting-associated protein 72 homolog|transcription factor-like 1|transformation suppressor gene YL-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on VPS72, check out the VPS72 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VPS72: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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