Product Info Summary
SKU: | PA2068 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Abl interactor 2 ABI2 Antibody
SKU/Catalog Number
PA2068
BA3031-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Abl interactor 2 ABI2 Antibody catalog # PA2068. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Abl interactor 2 ABI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2068)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ABI2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2068 is reactive to ABI2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
55663 MW
Background of ABI2
ABI2 (ABL Interactor 2), is a protein that in humans is encoded by the ABI2 gene. By analysis of a YAC and a BAC, Machado et al. (2000) mapped the ABI2 gene to 2q31-q33. ABI2 possesses a basic N terminus with homology to a homeodomain protein; a central serine-rich region; 3 PEST sequences, which are implicated in susceptibility to protein degradation; several proline-rich stretches; and an acidic C terminus with multiple phosphorylation sites and an SH3 domain. Dai and Pendergast (1995) suggested that the ABI proteins may function to coordinate the cytoplasmic and nuclear functions of the ABL1 tyrosine kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2068 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human 293T whole cell, human Hela whole cell, human K562 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABI2 using anti-ABI2 antibody (PA2068).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI2 antigen affinity purified polyclonal antibody (Catalog # PA2068) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABI2 at approximately 60 kDa. The expected band size for ABI2 is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ABI2 (Source: Uniprot.org, NCBI)
Gene Name
ABI2
Full Name
Abl interactor 2
Weight
55663 MW
Superfamily
ABI family
Alternative Names
Abl interactor 2;Abelson interactor 2;Abi-2;Abl-binding protein 3;AblBP3;Arg-binding protein 1;ArgBP1;ABI2;ARGBPIA; ABI2 ABI-2B, AIP-1, AIP1, AblBP3, SSH3BP2, argBP1, argBPIA, argBPIB, ABI2 abl interactor 2 abl interactor 2|abelson interactor 2|abl binding protein 3|abl-interacting protein 1 (SH3-containing protein)|abl-interactor protein 2b|arg protein tyrosine kinase-binding protein|arg-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABI2, check out the ABI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Abl interactor 2 ABI2 Antibody (PA2068)
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No publications found for PA2068
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Customer Q&As
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4 Customer Q&As for Anti-Abl interactor 2 ABI2 Antibody
Question
Is there a BSA free version of anti-ABI2 antibody PA2068 available?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ABI2 antibody PA2068 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-ABI2 antibody PA2068. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-24
Question
We are currently using anti-ABI2 antibody PA2068 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
The anti-ABI2 antibody (PA2068) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-10
Question
I was wanting to use your anti-ABI2 antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
You can see on the product datasheet, PA2068 anti-ABI2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-03