VEGF-E Vascular Endothelial Growth Factor-E Recombinant Protein(Orf Virus)

Product Name

VEGF-E Vascular Endothelial Growth Factor-E Recombinant Protein(Orf Virus)

View all VEGF recombinant proteins

SKU/Catalog Number

PROTQ2F842

Size

2ug, 10ug, 1mg

Description

A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.

Storage & Handling

Lyophilized Vascular Endothelial Growth Factor-E Orf Virus although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF E -OV should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Cite This Product

VEGF-E Vascular Endothelial Growth Factor-E Recombinant Protein(Orf Virus) (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ2F842)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Reconstitution

The lyophilized oVEGF-E Orf Virus should be reconstituted in water or medium to a concentration not lower than 50µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.

Biological Activity

The biological activity was determined (1) by the ability to induce VEGFR-2/KDR receptor phosphorylation in PAE/KDR cells and (2) in a cell proliferation assay using primary HUVECs. The ED₅₀ for this effect is typically 1-5ng/ml.

Reconstitution

The lyophilized oVEGF-E Orf Virus should be reconstituted in water or medium to a concentration not lower than 50µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.

Validation Images & Assay Conditions

Gene/Protein Information For VEGF (Source: Uniprot.org, NCBI)

Alternative Names

VEGF

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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