TNFSF18 (NM_005092) Human Over-expression Lysate

Product Name

TNFSF18 (NM_005092) Human Over-expression Lysate

See all GITR Ligand/TNFSF18 products

SKU/Catalog Number

LS008995

Size

100 µg

Description

Transient overexpression lysate of tumor necrosis factor (ligand) superfamily, member 18 (TNFSF18)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

TNFSF18 (NM_005092) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008995)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for TNFSF18 (Source: Uniprot.org, NCBI)

Alternative Names

Activation-inducible TNF-related ligand; AITRL; AITRLMGC138237; GITR Ligand; GITRL; GITRLglucocorticoid-induced TNFR-related protein ligand; Glucocorticoid-induced TNF-related ligand; hGITRLGITR ligand; TL6AITR ligand; TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18 TNFSF18 AITRL, GITRL, TL6, TNLG2A, hGITRL TNF superfamily member 18 tumor necrosis factor ligand superfamily member 18|AITR ligand|GITR ligand|activation-inducible TNF-related ligand|glucocorticoid-induced TNF-related ligand|glucocorticoid-induced TNFR-related protein ligand|tumor necrosis factor (ligand) superfamily, member 18|tumor necrosis factor ligand 2A|tumor necrosis factor superfamily member 18

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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