Product Info Summary
SKU: | LS064129 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of tubulointerstitial nephritis antigen-like 1 (TINAGL1) |
Customers Who Bought This Also Bought
Product info
Product Name
TINAGL1 (NM_022164) Human Over-expression Lysate
SKU/Catalog Number
LS064129
Size
100 µg
Description
Transient overexpression lysate of tubulointerstitial nephritis antigen-like 1 (TINAGL1)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
TINAGL1 (NM_022164) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS064129)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls064129 tinagl1 human over expression lysates nm 022164 wb testing 1 ls064129 tinagl1 human over expression lysates nm 022164 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls064129-tinagl1-human-over-expression-lysates-nm-022164-wb-testing-1.jpg)
Click image to see more details
TINAGL1 Human Over-expression Lysates NM_022164.
Western blot validation of overexpression lysate (LS064129) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TINAGL1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TINAGL1 (Source: Uniprot.org, NCBI)
Gene Name
TINAGL1
Full Name
Tubulointerstitial nephritis antigen-like
Weight
52.387kDa
Superfamily
peptidase C1 family
Alternative Names
androgen-regulated gene 1; ARG1; GIS5; Glucocorticoid-inducible protein 5; LCN7; LIECG3; lipocalin 7; OLRG2; OLRG-2; Oxidized LDL-responsive gene 2 protein; oxidized-LDL responsive gene 2; P3ECSL; TIN Ag-related protein; TINAGL; TINAGL1; TINAG-like 1; TINAGRP; TIN-ag-RP; tubulointerstitial nephritis antigen-like 1; tubulointerstitial nephritis antigen-like; Tubulointerstitial nephritis antigen-related protein TINAGL1 ARG1, LCN7, LIECG3, TINAGRP tubulointerstitial nephritis antigen like 1 tubulointerstitial nephritis antigen-like|OLRG-2|P3ECSL|TIN Ag-related protein|TIN-Ag-RP|TINAG-like 1|androgen-regulated gene 1|glucocorticoid-inducible protein 5|lipocalin 7|oxidized-LDL responsive gene 2|tubulointerstitial nephritis antigen-related protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TINAGL1, check out the TINAGL1 Infographic
![TINAGL1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TINAGL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TINAGL1 (NM_022164) Human Over-expression Lysate (LS064129)
Hello CJ!
No publications found for LS064129
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used TINAGL1 (NM_022164) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For TINAGL1 (NM_022164) Human Over-expression Lysate
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question