Click image to see more details
Product Info Summary
| SKU: | LS010494 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of serine/threonine kinase 25 (STE20 homolog, yeast) (STK25) |
Customers Who Bought This Also Bought
Product info
Product Name
STK25 (NM_006374) Human Over-expression Lysate
SKU/Catalog Number
LS010494
Size
100 µg
Description
Transient overexpression lysate of serine/threonine kinase 25 (STE20 homolog, yeast) (STK25)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
STK25 (NM_006374) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010494)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
STK25 Human Over-expression Lysates NM_006374.
Western blot validation of overexpression lysate (LS010494) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with STK25 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for STK25 (Source: Uniprot.org, NCBI)
Gene Name
STK25
Full Name
Serine/threonine-protein kinase 25
Weight
48.112kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; serine/threonine kinase 25 (Ste20, yeast homolog); serine/threonine kinase 25; serine/threonine-protein kinase 25; SOK-1; SOK1DKFZp686J1430; Ste20, yeast homolog; Ste20/oxidant stress response kinase 1; sterile 20 (oxidant stress response kinase 1; yeast Sps1/Ste20-related kinase1); Sterile 20/oxidant stress-response kinase 1; YSK1yeast) STK25 SOK1, YSK1 serine/threonine kinase 25 serine/threonine-protein kinase 25|Ste20, yeast homolog|ste20-like kinase|ste20/oxidant stress response kinase 1|sterile 20/oxidant stress-response kinase 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on STK25, check out the STK25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STK25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For STK25 (NM_006374) Human Over-expression Lysate (LS010494)
Hello CJ!
No publications found for LS010494
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used STK25 (NM_006374) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For STK25 (NM_006374) Human Over-expression Lysate
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
