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Product Info Summary
| SKU: | LS054908 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of coiled-coil domain containing 99 (CCDC99) |
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Product info
Product Name
Spindly (SPDL1) (NM_017785) Human Over-expression Lysate
SKU/Catalog Number
LS054908
Size
100 µg
Description
Transient overexpression lysate of coiled-coil domain containing 99 (CCDC99)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
Spindly (SPDL1) (NM_017785) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054908)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Spindly (SPDL1) Human Over-expression Lysates NM_017785.
Western blot validation of overexpression lysate (LS054908) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SPDL1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SPDL1 (Source: Uniprot.org, NCBI)
Gene Name
SPDL1
Full Name
Protein Spindly
Weight
Superfamily
Spindly family
Alternative Names
Arsenite-related gene 1 protein; coiled-coil domain containing 99; Coiled-coil domain-containing protein 99; FLJ20364; FLJ40690; hSpindly; protein Spindly; Rhabdomyosarcoma antigen MU-RMS-40.4A; rrhabdomyosarcoma antigen protein MU-RMS-40.4A; Spindly SPDL1 CCDC99 spindle apparatus coiled-coil protein 1 protein Spindly|arsenite-related gene 1 protein|coiled-coil domain-containing protein 99|rhabdomyosarcoma antigen MU-RMS-40.4A|rrhabdomyosarcoma antigen protein MU-RMS-40.4A
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SPDL1, check out the SPDL1 Infographic
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In this infographic, you will see the following information for SPDL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Spindly (SPDL1) (NM_017785) Human Over-expression Lysate (LS054908)
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