Protein Spindly Antibodies

AND SPDL1 ELISA kits, Protein Spindly Proteins

Many biological assays use antibodies to detect Protein Spindly, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Protein Spindly in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Protein Spindly antibodies...

We validate the specificity of these antibodies to Protein Spindly by testing them on tissues known to express SPDL1 positively and negatively. Browse below to find the SPDL1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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SPDL1 Infographic by Boster Bio

All SPDL1 Products

Product

Figures

Specificity

Applications

Price

Anti-CCDC99 SPDL1 Antibody

Human

ELISA, WB

$380
Cat # A12722
100ul

Spindly (SPDL1) (NM_017785) Human Over-expression Lysate

Human

$628
Cat # LS054908
100 µg

Anti-Spindly/SPDL1 Antibody Picoband™

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, ICC, WB

$370
Cat # A12722-2
100 µg/vial

SPDL1 Overview

Facts about Protein Spindly.

SPDL1 3D structure. Source: alphafold

Protein Spindly (SPDL1)

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is thought to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent creation of end-on kinetochore-microtubule attachments evidenced by the NDC80 complex.

Also required for proper spindle orientation. Does not seem to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment (PubMed:17576797, PubMed:19468067).

Gene Information

Human
Gene Name:	SPDL1
Uniprot:	Q96EA4
Entrez:	54908

Family

Belongs to:

Spindly family

Alternative Names

Arsenite-related gene 1 protein; coiled-coil domain containing 99; Coiled-coil domain-containing protein 99; FLJ20364; FLJ40690; hSpindly; protein Spindly; Rhabdomyosarcoma antigen MU-RMS-40.4A; rrhabdomyosarcoma antigen protein MU-RMS-40.4A; Spindly

Molecular Weight

Mass (kDA):
70.172 kDA

Genomic Context


Human

Location:	5q35.1
Sequence:	5; NC_000005.10 (169583661..169604778)

Subcellular Localizations

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.

Interacting Proteins

CA8
DNAAF4
PPP1R18
RTP5
TRAF4

USP15

PTMs

Acetylation

Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SPDL1

References

PMID: 17576797 by Griffis E.R., et al. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore.

PMID: 19468067 by Chan Y.W., et al. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.