Product Info Summary
SKU: | PROTP38507 |
---|---|
Size: | 10mg, 100mg, 1gram |
Source: | Escherichia coli |
Product info
Product Name
SPA Staphylococcal Protein-A 33.4kDa Recombinant Protein
View all SPA recombinant proteins
SKU/Catalog Number
PROTP38507
Size
10mg, 100mg, 1gram
Description
SPA Recombinant produced in E. coli is a homodimeric non-glycosylated polypeptide chains comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 296 amino acids and having a molecular mass of 33.4kDa containing little or no carbohydrate. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.
Storage & Handling
Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SPA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Cite This Product
SPA Staphylococcal Protein-A 33.4kDa Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP38507)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
SPA protein was lyophilized with no additives.
Purity
Greater than 97.0% as determined by (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Reconstitution
It is recommended to reconstitute the lyophilized SPA in sterile 18MΩ-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEED
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized SPA in sterile 18MΩ-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information For SPA (Source: Uniprot.org, NCBI)
Gene Name
SPA
Full Name
Weight
Alternative Names
Immunoglobulin G-binding protein A; IgG-binding protein A; Staphylococcal protein A; SPA
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SPA, check out the SPA Infographic
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In this infographic, you will see the following information for SPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For SPA Staphylococcal Protein-A 33.4kDa Recombinant Protein (PROTP38507)
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