$600 CUSTOM ANTIBODY Production Services

Svetlana Dzitoyeva

Research Scientist, Biochemistry and Molecular Genetics, University of Colorado, Denver

A Good Drosophila Me31B Antibody For WB

The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service.

Western Blot, Insect, D. melanogaster, ECL, Catalog# DZ33938-1

Erik de Vrieze

Postdoctoral Researcher, Dept of Otorhinolaryngology & Dept of Human Genetics, Radboud University Medical Center

Thank you for all the work on this project

First, we received our shipment of antibodies in perfect condition. Thank you for all the work on this project. On the left, you can find pictures of 5dpf wildtype and Ush2a mutant zebrafish retina stained with DZ01481 in red. The pictures show the typical layered retinal structure, with punctate Ush2a staining at the connecting cilium (cc). In blue, the nuclei of inner and outer nuclear layers are shown (INL/ONL).

Immunohistochemistry, Zebrafish retina 5dpf, Catalog# DZ01481

Jakub Famulski

Principal Investigator, Department of Biology, University of Kentucky

Good Zebrafish CDHR1a Antibody From Boster Bio

The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!

Immunofluorescence, Zebrafish embryos, 5 days old, Confocal Microscopy, Catalog# DZ07988

Jingli Cao

Assistant Professor, Weill Cornell Medical College

Good Zebrafish Mef2 Antibody

Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature.

Immunofluorescence, Zebrafish heart, Confocal Microscopy, Catalog# DZ01398-1

Jakub Famulski

Principal Investigator, Department of Biology, University of Kentucky

Good Zebrafish-Specific Siah1 Antibody From Boster Bio

Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!

Immunofluorescence, Zebrafish embryos 5dpf, Confocal Microscopy, Catalog# DZ02095

Hanjo Hellmann

Associate Professor, School of Biological Sciences, Washington State University

Verification Of Antibody On Recombinant Protein

We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.

Western Blot, Recombinant protein expressed in and purified from E. coli, Chemiluminescence, Catalog# DZ33980

Byron Baron

Principal Investigator & Research Academic and Lecturer, Centre for Molecular Medicine and Biobanking, Faculty of Medicine and Surgery, University of Malta

It worked relatively well

We have no alternative and it worked relatively well. The stability at room temperature is impressive considering the antibody took 3 weeks to be delivered.

Western blot, human and mouse whole cell lysates, Catalog# DZ41142

Yuzuru Imai

Associate Professor, Department of Research for Parkinson's Disease, Juntendo University Graduate School of Medicine

Western blotting is sufficient with this antibody

There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody.

Western blot, Drosophila melanogaster (head and thorax), Catalog# DZ41152

Erik de Vrieze

Postdoctoral Researcher, Radboud University Medical Center

Both Are Confirmed Specific In Zebrafish Using One Of Our KO Lines.

Ush2c/Adgrv1 was detected in a paraffin-embedded section of wildtype and KO mutant zebrafish retina tissue. The tissue section was blocked with 10% normal goat serum and 2% bovine serum albumin in PBS. The tissue section was then incubated with rabbit anti-Ush2c/Adgrv1 Antibody (DZ41032) at 1:100 overnight at 4°C. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:800 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Immunofluorescence,Anti-Zebrafish Ush2c/Adgrv1 N-Terminus Antibody, Confocal Microscopy, Catalog# DZ41032

Amin Ghabrial

Principal Investigator, Pathology and Cell Biology, Columbia University

The only antibody to Drosophila archease available and it works

My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining. Antibody staining reveals subcellular localization of the protein. In the salivary gland and trachea, localization was primarily nuclear. Polyclonal sera works with little background. May have to optimize staining. The bottom line: The only antibody to Drosophila archease available and it works.

Immunofluorescence, Drosophila larvae, Catalog# DZ33921-1

Erik de Vrieze

Postdoctoral Researcher, Radboud University Medical Center

Both Are Confirmed Specific In Zebrafish Using One Of Our KO Lines.

Ush2c/Adgrv1 was detected in a paraffin-embedded section of wildtype and KO mutant zebrafish retina tissue. The tissue section was blocked with 10% normal goat serum and 2% bovine serum albumin in PBS. The tissue section was then incubated with rabbit anti-Ush2c/Adgrv1 Antibody (DZ41033) at 1:100 overnight at 4°C. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:800 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Immunofluorescence,Anti-Zebrafish Ush2c/Adgrv1 N-Terminus Antibody, Confocal Microscopy, Catalog# DZ41033