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Product Info Summary
| SKU: | LS010283 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of CWC27 spliceosome-associated protein homolog (S. cerevisiae) (CWC27) |
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Product info
Product Name
SDCCAG10 (CWC27) (NM_005869) Human Over-expression Lysate
SKU/Catalog Number
LS010283
Size
100 µg
Description
Transient overexpression lysate of CWC27 spliceosome-associated protein homolog (S. cerevisiae) (CWC27)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
SDCCAG10 (CWC27) (NM_005869) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010283)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SDCCAG10 (CWC27) Human Over-expression Lysates NM_005869.
Western blot validation of overexpression lysate (LS010283) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CWC27 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CWC27 (Source: Uniprot.org, NCBI)
Gene Name
CWC27
Full Name
Spliceosome-associated protein CWC27 homolog
Weight
53.847kDa
Superfamily
cyclophilin-type PPIase family
Alternative Names
Antigen NY-CO-10; CWC27 spliceosome-associated protein homolog (S. cerevisiae); EC 5.2.1.8; NY-CO-10; peptidyl-prolyl cis-trans isomerase SDCCAG10; PPIase CWC27; PPIase SDCCAG10; SDCCAG10; Serologically defined colon cancer antigen 10peptidyl-prolyl cis-trans isomerase CWC27 homolog CWC27 NY-CO-10, RPSKA, SDCCAG-10, SDCCAG10 CWC27 spliceosome associated cyclophilin spliceosome-associated protein CWC27 homolog|CWC27 spliceosome associated protein homolog|PPIase CWC27|PPIase SDCCAG10| NY-CO-10|peptidyl-prolyl cis-trans isomerase CWC27 homolog|peptidyl-prolyl cis-trans isomerase SDCCAG10|probable inactive peptidyl-prolyl cis-trans isomerase CWC27 homolog|serologically defined colon cancer 10
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CWC27, check out the CWC27 Infographic
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In this infographic, you will see the following information for CWC27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SDCCAG10 (CWC27) (NM_005869) Human Over-expression Lysate (LS010283)
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