Product Info Summary
SKU: | RCOV02 |
---|---|
Size: | 100μg/vial |
Origin Species: | Mouse |
Source: | E. coli |
Product info
Product Name
SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag
SKU/Catalog Number
RCOV02
Size
100μg/vial
Description
SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag is produced in E. coli and the theoretical molecular weight is 22.7KD. This product is for research use only. Product is under validation for additional applications and indications. If you're interested, please contact [email protected].
Storage & Handling
The product is shipped at ambient temperature. Upon receipt, store it immediately at -20˚C for 6 months under sterile conditions. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Cite This Product
SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag (Boster Biological Technology, Pleasanton CA, USA, Catalog # RCOV02)
Form
Lyophilized
Formulation
Lyophilized from sterile 20mM PB, 150mM NaCl, PH 7.3-7.4, 10% glycerol and 4% trehalose
Purity
> 90%. Purification measurement method by SDS-PAGE quantitative densitometry by Coomassie® Blue Staining.
Method of purification: Nickel column affinity purification
Predicted MW
22.7KD
Endotoxin
Less than 1 EU/μg protein as determined by LAL method
Expression Form
In supernatant
Amino Acid Sequence
6×His tag at C-terminalAccession #: YP_009725304
AIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ
Background
Coronaviruses (CoV) are a family of large and enveloped positive-sense single-stranded RNA viruses that are classified into four genera, the alpha, beta, gamma, and delta coronaviruses. While gamma and delta coronaviruses mainly infect birds, alpha and beta coronaviruses are known to infect mammals and cause human respiratory illnesses such as the common cold, pneumonia, and severe diseases like SARS, MERS, and COVID-19. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, the N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.Assay dilution & Images
Validation Images & Assay Conditions
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Specific Publications For SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag (RCOV02)
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