Blocking Reagents

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  1. PBS Wash Buffer Pack AR1155
    $15.00
    • Western Blotting Reagents, IHC ICC IF Antibodies, Blocking Reagents
    • 1 pack (It is sufficient to make 500 ml of PBS wash buffer)
    • ELISA, IHC, WB
    • boster_tick.pngShips next business day
    Boster’s PBS Wash Buffer Pack is a pouch of dry-blend powder that is sufficient to make 500mL of PBS Wash Buffer for WB, ELISA and IHC. Learn More
  2. TBS Wash Buffer Pack AR0144
    $10.00
    • Western Blotting Reagents, IHC ICC IF Antibodies, Blocking Reagents
    • 1 pack (It is sufficient to make 500 ml of TBS wash buffer)
    • ELISA, IHC, WB
    • boster_tick.pngShips next business day
    Boster’s TBS Wash Buffer Pack is a pouch of dry-blend powder that is sufficient to make 500mL of TBS Wash Buffer for WB, ELISA and IHC. Learn More
  3. TBS Blocking Buffer Pack AR0143
    $15.00
    • Western Blotting Reagents, Blocking Reagents
    • 1 pack (It is sufficient to make 200 ml of TBS blocking buffer)
    • WB
    • boster_tick.pngShips next business day
    Boster’s TBS Blocking Buffer Pack is a pouch of dry-blend powder that is sufficient to make 200mL of TBS Blocking Buffer for Western blot. Learn More

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Western Blotting Membrane Blocking Products

membrane blocking

Introduction

Nitrocellulose membranes are ideal for blotting due to their ability to nonspecifically bind proteins. This allows your target proteins to bind the membrane and become immobilized, but it also would cause your antibodies to bind the entire membrane, producing no usable data. To prevent that from happening, the membrane must be saturated with a nonreactive protein to block the nonspecific binding of your primary and secondary antibodies.

Procedure

After completing the transfer of the protein sample to the membrane, thoroughly wash the membrane with Boster PBS wash buffer, then immerse the membrane in Boster TBS blocking buffer for 1-2 hours. Wash the membrane again to remove excess blocking buffer, and your membrane is ready for antibody incubation.

For a detailed protocol for membrane blocking see our Western blot handbook.

Tips

  1. Insufficient blocking can cause high background signal.
  2. Using nonfat milk blocking buffer can interfere with avadin-biotin detection systems
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