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Product Info Summary
| SKU: | DZ33938-1 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Fruit fly |
| Host: | Rabbit |
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Product info
Product Name
Anti-Fruit fly Me31B Antibody
SKU/Catalog Number
DZ33938-1
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-Me31B Antibody catalog # DZ33938-1. This antibody reacts with Fruit fly.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fruit fly Me31B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ33938-1)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
DZ33938-1 is reactive to me31B in Fruit fly
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Me31B using anti-Fruit fly Me31B antibody (DZ33938-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with non-fat dry milk for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fruit fly Me31B antibody (DZ33938-1) at 1:10,000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000, incubation 1h at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Me31B at approximately 52 kDa. The expected band size for Me31B is at 52 kDa.
Protein Target Info & Infographic
Gene/Protein Information For me31B (Source: Uniprot.org, NCBI)
Gene Name
me31B
Full Name
Weight
For more info on me31B, check out the me31B Infographic
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In this infographic, you will see the following information for me31B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fruit fly Me31B Antibody (DZ33938-1)
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Customer Reviews
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1 Reviews For Anti-Fruit fly Me31B Antibody
0
A Good Drosophila Me31B Antibody for WB--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist
Excellent
Source: Biocompare.com
| Application | Western Blot |
|---|---|
| Sample | Insect, D. melanogaster |
| Detection | ECL |
"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."
Svetlana Dzitoyeva
Verified customer
Submitted 2020-01-10
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