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Product Info Summary
| SKU: | DZ41152 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Fruit fly |
| Host: | Rabbit |
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Product info
Product Name
Anti-Fruit fly emp Antibody
SKU/Catalog Number
DZ41152
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-Fruit fly emp Antibody catalog # DZ41152. This antibody reacts with Fruit fly.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Fruit fly emp Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41152)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41152 is reactive to emp in Fruit fly
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Positive Control
WB: normal drosophila melanogaster head tissues, CG2727 KO / +, normal drosophila melanogaster thorax tissues, CG2727 KO / +
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Emp using anti-Emp antibody (DZ41152).
Electrophoresis was performed on a 7.5% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: normal drosophila melanogaster head tissues,
Lane 2: CG2727 KO / +,
Lane 3: normal drosophila melanogaster thorax tissues,
Lane 4: CG2727 KO / +.
After electrophoresis, proteins were transferred to PVDF at 20 V overnight. Blocked the membrane with 5% skim milk in PBS-T for 30 min at RT. The membrane was incubated with rabbit anti-Emp antigen affinity purified polyclonal antibody (Catalog # DZ41152) at 1:1000 dilution in PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times and probed with a goat anti-rabbit IgG-HRP secondary antibody at 1:10000 dilution PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times. The signal is detected with Immobilon Forte Western HRP substrate. A specific band was detected for Emp at approximately 59 kDa.
Protein Target Info & Infographic
Gene/Protein Information For emp (Source: Uniprot.org, NCBI)
Gene Name
emp
Full Name
Weight
For more info on emp, check out the emp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for emp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fruit fly emp Antibody (DZ41152)
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Customer Reviews
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1 Reviews For Anti-Fruit fly emp Antibody
0
Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine
Excellent
| Application | Western blot |
|---|---|
| Sample | Drosophila melanogaster (head and thorax) |
| Detection | Immobilon Forte Western HRP substrate |
"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."
Yuzuru Imai
Verified customer
Submitted 2021-04-29
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