Anti-Fruit fly emp Antibody

Product Name

Anti-Fruit fly emp Antibody

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SKU/Catalog Number

DZ41152

Size

200 μl/vial

Form

Liquid

Description

Boster Bio Polyclonal Anti-Fruit fly emp Antibody catalog # DZ41152. This antibody reacts with Fruit fly.

Storage & Handling

At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.

Cite This Product

Anti-Fruit fly emp Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41152)

Host

Rabbit

Contents

Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

DZ41152 is reactive to in Fruit fly

Applications

DZ41152 is guaranteed for Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Emp using anti-Emp antibody (DZ41152).
Electrophoresis was performed on a 7.5% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: normal drosophila melanogaster head tissues,
Lane 2: CG2727 KO / +,
Lane 3: normal drosophila melanogaster thorax tissues,
Lane 4: CG2727 KO / +.
After electrophoresis, proteins were transferred to PVDF at 20 V overnight. Blocked the membrane with 5% skim milk in PBS-T for 30 min at RT. The membrane was incubated with rabbit anti-Emp antigen affinity purified polyclonal antibody (Catalog # DZ41152) at 1:1000 dilution in PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times and probed with a goat anti-rabbit IgG-HRP secondary antibody at 1:10000 dilution PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times. The signal is detected with Immobilon Forte Western HRP substrate. A specific band was detected for Emp at approximately 59 kDa.

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