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Product Info Summary
| SKU: | DZ33980 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Baker's yeast |
| Host: | Rabbit |
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Product info
Product Name
Polyclonal Anti-BnaA07g20720D Antibody
SKU/Catalog Number
DZ33980
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-BnaA07g20720D Antibody catalog # DZ33980. This antibody reacts with No validated species.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Polyclonal Anti-BnaA07g20720D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ33980)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ33980 is reactive to in Baker's yeast
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BnaA07g20720D using anti-BnaA07g20720D antibody (DZ33980).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BnaA07g20720D antigen affinity purified polyclonal antibody (Catalog # DZ33980) at 1:5000 for 90 min at room temperature, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 90 min at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Specific Publications For Polyclonal Anti-BnaA07g20720D Antibody (DZ33980)
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Customer Reviews
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1 Reviews For Polyclonal Anti-BnaA07g20720D Antibody
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Verification of Antibody on Recombinant Protein--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor
Excellent
Source: Biocompare.com
| Application | Western Blot |
|---|---|
| Sample | Recombinant protein expressed in and purified from E. coli |
| Detection | Chemiluminescence |
"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."
Hanjo Hellmann
Verified customer
Submitted 2020-01-10
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