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Product Info Summary
| SKU: | LS006199 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2) |
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Product info
Product Name
P70 S6 Kinase beta (RPS6KB2) (NM_003952) Human Over-expression Lysate
See all p70 S6 Kinase beta/S6K2 products
SKU/Catalog Number
LS006199
Size
100 µg
Description
Transient overexpression lysate of ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
P70 S6 Kinase beta (RPS6KB2) (NM_003952) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006199)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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P70 S6 Kinase beta (RPS6KB2) Human Over-expression Lysates NM_003952.
Western blot validation of overexpression lysate (LS006199) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPS6KB2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RPS6KB2 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KB2
Full Name
Ribosomal protein S6 kinase beta-2
Weight
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; KLS; p70 ribosomal S6 kinase beta; p70 S6 Kinase beta; p70 S6KB; p70 S6K-beta; p70(S6K)-beta; p70-beta; P70-beta-1; P70-beta-2; p70-S6K 2; P70S6K2; p70S6Kb; ribosomal protein S6 kinase beta-2; ribosomal protein S6 kinase, 70kD, polypeptide 2,70 kDa ribosomal protein S6 kinase 2; ribosomal protein S6 kinase, 70kDa, polypeptide 2; RPS6KB2; S6K2; S6K-beta; S6K-beta2; S6K-beta-2; serine/threonine-protein kinase 14 beta; Serine/threonine-protein kinase 14B; SRK; STK14B; STK14BS6 kinase-related kinase RPS6KB2 KLS, P70-beta, P70-beta-1, P70-beta-2, S6K-beta2, S6K2, S6KB, S6KI(2), S6Kbeta, SRK, STK14B, p70(S6K)-beta, p70S6Kb ribosomal protein S6 kinase B2 ribosomal protein S6 kinase beta-2|70 kDa ribosomal protein S6 kinase 2|P70S6K2|S6 kinase-related kinase|S6K-beta|S6K-beta-2|p70 S6 kinase beta|p70 S6K-beta|p70 S6KB|p70 ribosomal S6 kinase beta|p70-S6K 2|ribosomal protein S6 kinase, 70kDa, polypeptide 2|serine/threonine-protein kinase 14 beta|serine/threonine-protein kinase 14B
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RPS6KB2, check out the RPS6KB2 Infographic
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In this infographic, you will see the following information for RPS6KB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For P70 S6 Kinase beta (RPS6KB2) (NM_003952) Human Over-expression Lysate (LS006199)
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