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Product Info Summary
| SKU: | EK7116 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | All species |
| Application: | ELISA |
| Sample Types: | Plasma, Serum, Cell Lysates, Urine |
Product info
Product Name
Nitrotyrosine ELISA Kit
See all Nitrotyrosine products
SKU/Catalog Number
EK7116
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Colorimetric detection of Nitrotyrosine. 96 wells/kit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Nitrotyrosine ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7116)
Clonality of Antibodies
See Datasheet for details
Sensitivity
50 nM
Assay Range
62.5 - 8000 nM
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7116 is reactive to Nitrotyrosine in All species samples
Validated Sample Types
Plasma, Serum, Cell Lysates, Urine
Application Guarantee
EK7116 is guaranteed for ELISA in All species by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Nitrosylated BSA Coated Plate | 1 Plate |
| Nitrotyrosine Standard | 1 vial/110µl |
| Nitrotyrosine HRP Conjugated Monoclonal Antibody | 1 vial/75µl |
| Sample and Standard Diluent (Red) | 1 vial/50mL |
| Nitrotyrosine Antibody Diluent (Blue) | 1 vial/13mL |
| Wash Buffer Concentrate (10X) | 1 vial/ 50mL |
| TMB Substrate | 1 vial/ 13 ml |
| Stop Solution | 1 vial/ 13 ml |
| Plate Cover | 2 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Diagram of the Triplicate Sample Plate Format
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Diagram of the Competitive ELISA
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Diagram of the Preparation of Nitrotyrosine Standards
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Typical Standard Curve for the Nitrotyrosine ELISA Kit (Enzyme-Linked Immunosorbent Assay)K7116
Assay Type: Competitive ELISA.
Detection Method: Colorimetric Assay.
Assay Range: 62.5 8000 nM.
Specific Publications For Nitrotyrosine ELISA Kit (EK7116)
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