NF-kappaB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit

Product Name

NF-kappaB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit

See all RELA products

SKU/Catalog Number

EKC2478

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The NF-kappaB p65 (Phospho-Thr254) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NF-kappaB p65 (Phospho-Thr254) protein expression profile in cells. The kit can be used for measuring the relative amounts of NF-kappaB p65 (Phospho-Thr254) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NF-kappaB p65 (Phospho-Thr254).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

NF-kappaB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2478)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2478 is reactive to RELA in Human, Mouse, Rat samples

Application Guarantee

EKC2478 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent	Quantity	Container
96-Well Cell Culture Clear-Bottom Microplate	2 Plates	-
10x TBS	24 ml (10x)	Clear
Quenching Buffer	24 ml (1x)	Clear
Blocking Buffer	50 ml (1x)	Clear
15x Wash Buffer	50 ml (15x)	Clear
100x Anti-NF-kappaB p65 Phospho-Thr254 Antibody (Rabbit Polyclonal)	60 µl (100x)	Red
100x Anti-NF-kappaB p65 Antibody (Rabbit Polyclonal)	60 µl (100x)	Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal)	60 µl (100x)	Green
HRP-Conjugated Anti-Rabbit IgG Antibody	12ml (1x)	Glass
HRP-Conjugated Anti-Mouse IgG Antibody	12ml (1x)	Glass
Primary Antibody Diluent	12 ml (1x)	Clear
Ready-to-Use Substrate	12 ml (1x)	Brown
Stop Solution	12 ml (1x)	Clear
Crystal Violet Solution	12ml (1x)	Glass
SDS Solution	24 ml (1x)	Clear
Adhesive Plate Seals	2 Seals	-

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
• Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
• 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
• Deionized or sterile water
• Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
• Graph paper or computer software capable of generating or displaying logarithmic functions
• Absorbent papers or vacuum aspirator
• Test tubes or microfuge tubes capable of storing 1 ml
• Orbital shaker
• Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For RELA (Source: Uniprot.Org, NCBI)

Alternative Names

Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;RELA;NFKB3; RELA CMCU, NFKB3, p65 RELA proto-oncogene, NF-kB subunit transcription factor p65|NF-kappa-B p65delta3|NF-kappa-B transcription factor p65|nuclear factor NF-kappa-B p65 subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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