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Flow Cytometry
FACS Flow Cytometry Technical Blogs
*Note to educators: you are permitted to share BosterBio's resources and PDFs on your class websites and lab websites. Please make sure to cite or link to the origin.
6 Helpful Tips FACS Multicolor Panel Design
October 27, 2017
Keywords: flow cytometry, FACS antibodies, multicolor panels, CD antibodies, resources, fluorophores
Planning to design multicolor panels for FACS antibodies? Boster presents 6 helpful tips to keep in mind when designing multicolor flow cytometry panels! Don’t miss the FACS resources and recommendations we’ve compiled in this newsletter!
What are FMO controls? 3 recommendations...
September 26, 2017
Keywords: flow cytometry experimental controls, fluorescence minus one staining control, FACS analysis tips and tricks, technical recommendations and complete downloadable ebook, immunology, cell biology products catalog, virtual event
There are 3 situations when an FMO control is highly recommended! The Fluorescence Minus One (FMO controls) are staining controls that contain all the antibodies of a panel minus 1 of them. It measures the spillover of all those other fluorophores in the channel of the missing antibody, and is used to identify and gate cells in the context of data spread due to the multiple fluorophores. It is a stronger negative control than the regular unstained control as it takes into account how the other stains in the panel impact the channel that is left out. Read on for technical tips and tricks when it comes to flow cytometry (FACS).
The Why’s and How’s of Counting Cells for FACS
June 30, 2017
Keywords: Flow cytometry tips, counting cells principle explanation, FACS protocol cell count, how to count cells, hemocytometer
Counting cells before the staining procedure and the analysis/live sort is not up for debate! Cell numbers affect the staining quality, the FACS instrument reading, as well as the efficacy of any downstream assay, in case the cells are live sorted. Furthermore, cells are steadily lost during the staining procedure and it is therefore imperative that enough cells are available at the start of the experiment to compensate for the inevitable losses. There are various methods available for counting cells which are explained in this technical newsletter.
5 Ingredients to Consider in FACS Buffer
May 12, 2017
Keywords: check list, recipe, FACS buffer ingredients, Flow cytometry, cell sorting preparation
If you perform flow cytometry, these recipe tips for FACS Buffers may assist you! We recommend these ingredients to improve your cell sorting process.
3 Guidelines for Using Secondary Antibodies
Apr 16, 2017
Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies
The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.
Mini Guide to FACS Staining Controls
Mar 31, 2017
Keywords: table of controls, technical tips, FACS, Flow cytometry, cell sorting preparation, recommended experimental controls
With today's selection of fluorochromes, FACS panels can become quite complex. Working with multiple colors and antibodies will require the appropriate experimental controls in order to properly interpret the data results. Check to make sure you have included all the appropriate controls before your cell sorting experiment! This convenient mini guide to FACS staining controls by Dr. Booster should be a helpful reference tool.
FACS Sorting Preparation Checklist
Mar 20, 2017
Keywords: check list, technical tips, FACS, Flow cytometry, cell sorting preparation
Are you preparing for a FACS experiment? Here’s a 10 point checklist to help you get ready prior to cell sorting. We suggest going through this checklist beforehand, as your cell sorting process may go more quickly and efficiently if you read the following tips.
Troubleshooting Guides
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Troubleshooting GuidesRelated Pages
Mini Guide to FACS Staining Controls
Newsletter Mini Guide to FACS Staining Controls *Note to educators: you are permitted to share BosterBio's resources and PDFs on your class websites and lab websites. Troubleshooting guides Dear Earthling, With great versatility (in FACS panels and p...
See More5 Ingredients to Consider in FACS Buffer
5- Add sodium azide (0.1-1%) Sodium azide – at suitable low concentrations – checks bacterial contamination, prevents photo-bleaching of fluorchromes and blocks antibody shedding. Troubleshooting Guides Download troubleshootingnhandbooks for IHC,...
See MoreBoster Technical Blogs and News
FACS Sorting Preparation Checklist Mar 20 2017 Keywords: check list, technical tips, FACS, Flow cytometry, cell sorting preparation Are you preparing for a FACS experiment? 6 Helpful Tips FACS Multicolor Panel Design Oct 27 2017 Keywords: flow cytome...
See MoreCounting Cells for FACS
The four corner squares of the grid measure 1 mm x 1 mm and each square is further subdivided into 0.05 mm x 0.05 mm squares. The Why's and How's of Counting Cells for FACS Greetings Earthling, Counting cells before the staining procedure and the...
See MoreHelpful Tips FACS Multicolor Panel Design
Newsletter Your Monthly Signaling Pathway Posters *Note to educators: you are permitted to share BosterBio's resources and PDFs on your class websites and lab websites. It is also advisable to use FMO controls (see our FACS resources for details) to ...
See MoreWhat are FMO controls? 3 recommendations...
Detection of rare or complex cell sub-populations through a multicolor panel requires very precise gating, the limits of which can be accurately set by utilizing the FMO controls. Troubleshooting guides Greetings Earthling, The Fluorescence Minus One...
See MoreFlow Cytometry FACS Troubleshooting Guide Download Page
This guide will show you all the nuts and blots for Flow Cytometry and FACS, including expert review of principle, optimized protocol that really works and more. Comprehensive Troubleshooting Tips Having our troubleshooting tips that address all kind...
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