NALP2 (NLRP2) (NM_017852) Human Over-expression Lysate

Lysate of NLRP2/NALP2 over-expression cell lines, WB control

Transient overexpression lysate of NLR family, pyrin domain containing 2 (NLRP2)

Product Info Summary

SKU: LS055655
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of NLR family, pyrin domain containing 2 (NLRP2)

Product Name

NALP2 (NLRP2) (NM_017852) Human Over-expression Lysate

See all NLRP2/NALP2 products

SKU/Catalog Number

LS055655

Size

100 µg

Description

Transient overexpression lysate of NLR family, pyrin domain containing 2 (NLRP2)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

NALP2 (NLRP2) (NM_017852) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS055655)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for NLRP2 (Source: Uniprot.org, NCBI)

Gene Name

NLRP2

Full Name

NACHT, LRR and PYD domains-containing protein 2

Weight

120.515kDa

Superfamily

NLRP family

Alternative Names

CLR19.9; FLJ20510; NACHT, leucine rich repeat and PYD containing 2; NACHT, LRR and PYD domains-containing protein 2; NALP2; NALP2NACHT, LRR and PYD containing protein 2; NBS1; NBS1PYRIN-containing APAF1-like protein 2; NLR family, pyrin domain containing 2; NLRP2; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 2; PAN1; PAN1Nucleotide-binding site protein 1; PYPAF2; PYPAF2PYRIN domain and NACHT domain-containing protein 1 NLRP2 CLR19.9, NALP2, NBS1, PAN1, PYPAF2 NLR family pyrin domain containing 2 NACHT, LRR and PYD domains-containing protein 2|NACHT, leucine rich repeat and PYD containing 2|PYRIN domain and NACHT domain-containing protein 1|PYRIN-containing APAF1-like protein 2|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 2|nucleotide-binding site protein 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on NLRP2, check out the NLRP2 Infographic

NLRP2 infographic

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In this infographic, you will see the following information for NLRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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