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Product Info Summary
| Species: | Mouse |
|---|---|
| Sample Type: | Serum, Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 5.5 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Catalog: | MEK1002 |
| Manufacturer Reference Number: | 112149MS-15P |
| Analytes: | GMCSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, MIP-1α, RANTES, TNFα |
Product info
Product Overview
The Multiplex Mouse Cytokine Stripwells (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using just 25 µl of sample per well, up to 80 samples can be assayed for all 15 markers in the panel within 5.5 hours.
The Multiplex Mouse Cytokine Stripwells (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection |
|---|---|---|---|---|---|
| GMCSF | Sandwich | 560 - 0.77 (pg/mL) | 392 (pg/mL) | 1 (pg/mL) | 0.38 (pg/mL) |
| IFNγ | Sandwich | 516 - 0.71 (pg/mL) | 361 (pg/mL) | 0.94 (pg/mL) | 0.35 (pg/mL) |
| IL-1α | Sandwich | 610 - 0.84 (pg/mL) | 427 (pg/mL) | 1.1 (pg/mL) | 0.42 (pg/mL) |
| IL-1β | Sandwich | 2500 - 3.43 (pg/mL) | 1750 (pg/mL) | 4.5 (pg/mL) | 1.71 (pg/mL) |
| IL-2 | Sandwich | 360 - 0.49 (pg/mL) | 252 (pg/mL) | 0.6 (pg/mL) | 0.25 (pg/mL) |
| IL-3 | Sandwich | 60 - 0.08 (pg/mL) | 42 (pg/mL) | 0.1 (pg/mL) | 0.04 (pg/mL) |
| IL-4 | Sandwich | 400 - 0.55 (pg/mL) | 280 (pg/mL) | 0.7 (pg/mL) | 0.27 (pg/mL) |
| IL-5 | Sandwich | 546 - 0.75 (pg/mL) | 382 (pg/mL) | 0.96 (pg/mL) | 0.37 (pg/mL) |
| IL-6 | Sandwich | 504 - 0.69 (pg/mL) | 353 (pg/mL) | 0.91 (pg/mL) | 0.35 (pg/mL) |
| IL-10 | Sandwich | 667 - 0.91 (pg/mL) | 467 (pg/mL) | 1.33 (pg/mL) | 0.46 (pg/mL) |
| IL-12p70 | Sandwich | 572 - 0.78 (pg/mL) | 400 (pg/mL) | 1.01 (pg/mL) | 0.39 (pg/mL) |
| IL-17A | Sandwich | 760 - 1.04 (pg/mL) | 532 (pg/mL) | 1.4 (pg/mL) | 0.52 (pg/mL) |
| MIP-1α | Sandwich | 1000 - 1.37 (pg/mL) | 700 (pg/mL) | 1.8 (pg/mL) | 0.69 (pg/mL) |
| RANTES | Sandwich | 1653 - 2.27 (pg/mL) | 1157 (pg/mL) | 2.92 (pg/mL) | 1.13 (pg/mL) |
| TNFα | Sandwich | 429 - 0.59 (pg/mL) | 300 (pg/mL) | 0.69 (pg/mL) | 0.29 (pg/mL) |
Kit Components
Each kit contains a 96-well plate, with relevant biomarker panel in each well, and contains all reagents required to perform testing.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
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2 Customer Q&As for Multiplex ELISA Kit For Mouse Cytokine - Stripwells (15-plex)
Question
What is a recommended reader for MEK1002? If not, is this product available with multiplex reader?
Verified customer
Asked: 2020-01-13
Answer
For the Multiplex ELISA Kit For Mouse Cytokine - Stripwells (16-Plex) (MEK1002), we highly recommend the Quansys Q-Plex reader because we can guarantee results on the Q-Plex imager. Some third party imagers can be used, but we do not support them because we do not validate them.
Boster Scientific Support
Answered: 2020-01-22
Question
How does MEK1002 kit differ from MEK1006 kit if both have the same target proteins and 96-well solid plate?
Verified customer
Asked: 2019-08-13
Answer
The Multiplex ELISA Kit For Mouse Cytokine - Stripwells (16-plex) MEK1002 are manufactured for the purpose of multiple usages, the kit has separable strips and comes with three calibrator vials from the same lot and extra plate seals. The Multiplex ELISA Kit For Mouse Cytokine - Screen (16-plex) MEK1006 on the other hand has a solid plate and is meant for one time use only.
Boster Scientific Support
Answered: 2019-08-13
