Mouse WISP1/CCN4 ELISA Kit PicoKine®

Product Name

Mouse WISP1/CCN4 ELISA Kit PicoKine®

View all WISP-1/CCN4 ELISA kits

SKU/Catalog Number

EK1195

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse WISP1/CCN4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccn4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse WISP1/CCN4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1195)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: T23-N367

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

See datasheet of EK1195 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1195 is reactive to Ccn4 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1195 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of WISP-1/CCN4

WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

Kit Components

Catalog Number	Description	Quantity
EK1195-CAP	Anti-Mouse Ccn4 Pre-coated 96-well strip microplate	1
EK1195-ST	Mouse Ccn4 Standard	2 vials, 10 ng/tube
EK1195-DA	Mouse Ccn4 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.067	0.256	0.320	0.499	0.925	1.385	1.947	2.393

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect WISP-1/CCN4, Dilution ratio of 1:1, concentration in serum and plasma is 1-2 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse WISP1/CCN4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of WISP-1/CCN4 in ELISA kits from four different production batches/lots.

Gene/Protein Information For Ccn4 (Source: Uniprot.Org, NCBI)

Alternative Names

WNT1-inducible-signaling pathway protein 1;WISP-1;CCN family member 4;ELM-1;Wisp1;Ccn4, Elm1; CCN4 WISP1, WISP1-OT1, WISP1-UT1, WISP1c, WISP1i, WISP1tc cellular communication network factor 4 CCN family member 4|WISP1 3UTR-associated RNA 1|WISP1 overlapping transcript 1 (non-protein coding)|WNT1 induced secreted protein 1|WNT1 inducible signaling pathway protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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