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Product Info Summary
| SKU: | EK0772 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse SPINT1/HAI-1 ELISA Kit PicoKine®
View all HAI-1/HGFA Inhibitor 1 ELISA kits
SKU/Catalog Number
EK0772
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SPINT1/HAI-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Spint1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SPINT1/HAI-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0772)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E30-L507
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0772 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0772 is reactive to Spint1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0772 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HAI-1/HGFA Inhibitor 1
Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0772-CAP | Anti-Mouse Spint1 Pre-coated 96-well strip microplate | 1 |
| EK0772-ST | Mouse Spint1 Standard | 2 vials, 10 ng/tube |
| EK0772-DA | Mouse Spint1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
| O.D. | 0.007 | 0.084 | 0.165 | 0.304 | 0.585 | 0.93 | 1.636 | 2.225 |
Data Example Images
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Mouse SPINT1/HAI-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SPINT1/HAI-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 199 | 707 | 1860 | 185 | 728 | 1735 |
| Standard deviation | 14.72 | 41 | 119.04 | 16.4%1 | 48.1%3 | 140.53 |
| CV (%) | 7.4% | 5.8% | 6.4% | 8.6% | 6.2% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HAI-1/HGFA Inhibitor 1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 199 | 180 | 196 | 188 | 190 | 7.39 | 3.8% |
| Sample 2 | 707 | 699 | 666 | 710 | 695 | 17.5 | 2.5% |
| Sample 3 | 1860 | 1696 | 2012 | 1755 | 1830 | 120 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For Spint1 (Source: Uniprot.Org, NCBI)
Gene Name
Spint1
Full Name
Kunitz-type protease inhibitor 1
Weight
56591 MW
Alternative Names
Kunitz-type protease inhibitor 1;Hepatocyte growth factor activator inhibitor type 1;HAI-1;Spint1;Hai1; SPINT1 HAI, HAI1, MANSC2 serine peptidase inhibitor, Kunitz type 1 kunitz-type protease inhibitor 1|HAI-1|hepatocyte growth factor activator inhibitor type 1|serine protease inhibitor, Kunitz type 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Spint1, check out the Spint1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Spint1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SPINT1/HAI-1 ELISA Kit PicoKine® (EK0772)
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Customer Q&As
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7 Customer Q&As for Mouse SPINT1/HAI-1 ELISA Kit PicoKine®
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
W. Mehta
Verified customer
Asked: 2020-04-22
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-22
Question
Q: if the enzyme conjugated HAI-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the HAI-1 antigen?
Y. Anand
Verified customer
Asked: 2020-02-15
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-02-15
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification HAI-1 using the EK0772 Mouse HAI-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-07-28
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-07-28
Question
Q: What is the optimal O.D. value for HAI-1 ELISA kit? I performed your HAI-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain HAI-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this HAI-1 ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of HAI-1 that can be declared/interpreted as positive by the above standard is 12pg/ml.
Boster Scientific Support
Answered: 2019-06-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-01-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-09-29
Question
Q: can I use citrate plasma as samples in Mouse HAI-1 Picokine® ELISA Kit (Catalog # EK0772)?
M. Adams
Verified customer
Asked: 2017-04-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of HAI-1 causing disruption of its protein structure. HAI-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the HAI-1 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-04-29
