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Product Info Summary
| SKU: | PROTP97463 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Mouse recombinant Neurturin protein, AF
View all Neurturin recombinant proteins
SKU/Catalog Number
PROTP97463
Size
5ug,20ug,100ug
Tag
His Tag (N-term)
Description
Neurturin is a 22 kDa cytokine with 195 amino acid residues. Neurturin belongs to the same neurotrophic factor family as GDNF. Binding to GFRα-2 (GPI-linked receptor termed GDNF family receptor α-2), neurturin activates Ret receptor tyrosine kinase and MAPK pathway. It involves in neuron survival.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Mouse recombinant Neurturin protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP97463)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
22.405kDa
Molecular weight
The protein has a calculated MW of 12.33 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce proliferation in SH-SY5Y cells. The ED₅₀ for this effect is <50 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
PGARPCGLRELEVRVSELGLGYTSDETVLFRYCAGACEAAIRIYDLGLRRLRQRRRVRRERARAHPCCRPTAYEDEVSFLDVHSRYHTLQELSARECACV with polyhistidine tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant mouse Neurturin
Protein Target Info & Infographic
Gene/Protein Information For Nrtn (Source: Uniprot.org, NCBI)
Gene Name
Nrtn
Full Name
Neurturin
Weight
22.405kDa
Superfamily
TGF-beta family
Alternative Names
NTN, NRTN NRTN NTN neurturin neurturin|prepro-neurturin
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Nrtn, check out the Nrtn Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nrtn: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Mouse recombinant Neurturin protein, AF (PROTP97463)
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