Anti-Neurturin NRTN Antibody

Product Name

Anti-Neurturin NRTN Antibody

View all Neurturin Antibodies

SKU/Catalog Number

A05924

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Neurturin NRTN Antibody (Catalog # A05924). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Neurturin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Neurturin NRTN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05924)

Host

Rabbit

Contents

Neurturin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Neurturin antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Neurturin. The immunogen is located within the last 50 amino acids of Neurturin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.

Reactive Species

A05924 is reactive to NRTN in Human, Mouse

Applications

A05924 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

22405 MW

Background of Neurturin

Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently and termed GDNFRβ, Ret ligand 2 (RETL2) or TGF-beta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. NTN binds to and forms a complex with GFRα-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Neurturin antibody can be used for detection of neurturin by Western blot. An approximate 14 kDa band of the full length recombinant NTN was detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.

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Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 μg/mL.

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Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.

Green: Neuriturin Antibody (A05924)
Red: Phylloidin staining
Blue: DAPI staining

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Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.

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Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibody at 5 μg/mL.

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Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibody at 20 μg/mL.

Green: Neuriturin Antibody (A05924)
Red: Phylloidin staining
Blue: DAPI staining

Gene/Protein Information For NRTN (Source: Uniprot.org, NCBI)

Alternative Names

Neurturin; NRTN; NTN NRTN NTN neurturin neurturin|prepro-neurturin

For more info on NRTN, check out the NRTN Infographic

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In this infographic, you will see the following information for NRTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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