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Product Info Summary
| SKU: | PROTP04401-4 |
|---|---|
| Size: | 5ug,20ug,100ug,500ug,1mg |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Mouse recombinant IL-5 (Interleukin-5) protein, AF
View all IL-5 recombinant proteins
SKU/Catalog Number
PROTP04401-4
Size
5ug,20ug,100ug,500ug,1mg
Tag
His Tag (C-term)
Description
Interleukin 5 (IL-5) is a 45-60 kDa homodimeric cytokine that functions principally in the eosinophil arm of the Th2 response. It is a member of the helical bundle family of cytokines, along with IL-3, IL-4, and granulocyte macrophage-colony stimulating factor (GM-CSF).
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Mouse recombinant IL-5 (Interleukin-5) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP04401-4)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
15.238kDa
Molecular weight
The protein has a calculated MW of 13.93 kDa. The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant mouse IL-5 is > 5 x 10⁶ IU/mg.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MEIPMSTVVKETLTQLSAHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEMLFQNLSLIKKYIDRQKEKCGEERRRTRQFLDYLQEFLGVMSTEWAMEG with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant mouse IL-5
Protein Target Info & Infographic
Gene/Protein Information For IL5 (Source: Uniprot.org, NCBI)
Gene Name
IL5
Full Name
Interleukin-5
Weight
15.238kDa
Superfamily
IL-5 family
Alternative Names
EDF, BCDFII, TRF IL5 EDF, IL-5, TRF interleukin 5 interleukin-5|B-cell differentiation factor I|T-cell replacing factor|colony-stimulating factor, eosinophil|eosinophil differentiation factor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL5, check out the IL5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Mouse recombinant IL-5 (Interleukin-5) protein, AF (PROTP04401-4)
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