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Product Info Summary
| SKU: | PROTP51859 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Mouse recombinant HDGF (Hepatoma-derived growth factor) protein, AF
View all HDGF recombinant proteins
SKU/Catalog Number
PROTP51859
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Heparin Binding Growth Factors (HDGF) is a 27.6 kDa hepatoma-derived Growth Factors with 246 amino acid residues. HDGF is mainly expressed from mitochondria and proteasome. Functionally, it is a potent mitogen, stimulating the growth of vascular smooth muscle cells, hepatoma cells and endothelial cells, acting as a transcriptional repressor. HDGF is expressed in a variety of cancers.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Mouse recombinant HDGF (Hepatoma-derived growth factor) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP51859)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
26.788kDa
Molecular weight
The protein has a calculated MW of 27.08 kDa. The protein migrates as 35-48 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant mouse HDGF
Protein Target Info & Infographic
Gene/Protein Information For Hdgf (Source: Uniprot.org, NCBI)
Gene Name
Hdgf
Full Name
Hepatoma-derived growth factor
Weight
26.788kDa
Superfamily
HDGF family
Alternative Names
AI118077, D3Ertd299, D3Ertd299e HDGF HMG1L2 heparin binding growth factor hepatoma-derived growth factor|epididymis secretory sperm binding protein|hepatoma derived growth factor|high mobility group protein 1-like 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Hdgf, check out the Hdgf Infographic
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In this infographic, you will see the following information for Hdgf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Mouse recombinant HDGF (Hepatoma-derived growth factor) protein, AF (PROTP51859)
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