Key=store_id
Key=entity_id, value=47919
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Key=sku, value=EK1266
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Key=created_at, value=2018-11-29 14:57:36
Key=updated_at, value=2024-10-19 05:05:39
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Mouse Myostatin/GDF8 ELISA Kit PicoKine®
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Key=price, value=499.0000
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Key=meta_title, value=Mouse Myostatin/GDF8 ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Mouse Myostatin/GDF8 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.
Key=image, value=/e/k/ek1266.png
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Key=url_key, value=mouse-myostatin-gdf8-picokine-trade-elisa-kit-ek1266-boster
Key=url_path, value=mouse-myostatin-gdf8-picokine-trade-elisa-kit-ek1266-boster
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Key=image_label, value=Mouse Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
Key=small_image_label, value=Mouse Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
Key=thumbnail_label, value=Mouse Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
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Key=size, value=96 wells/kit, with removable strips.
Key=gene_full_name, value=myostatin
Key=uniprot_id, value=O08689
Key=assay_range, value=31.2 pg/ml - 2,000 pg/ml
Key=sensitivity, value=<10 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Mouse
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=A monoclonal antibody from rat|a biotinylated detection polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=predicted_reactivity
Key=source_company, value=Boster EK1266
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Neuroscience, Signal Transduction
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Key=review_count, value=8
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Key=cj_related_products, value=Muscle Fiber Hypertrophy:EK1265|EK1267|A00926|A00926-2^Satellite Cell Proliferation:EK1267
Key=description, value=Mouse Myostatin/GDF8 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Mouse Myostatin/GDF8 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=background, value=Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 1 C1.1; 1 26.99 cM. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.
Key=gene_name, value=MSTN
Key=synonyms, value=Growth/differentiation factor 8; GDF-8; Myostatin; Mstn; Gdf8
Key=sample_type, value=cell culture supernatants, tissue, serum and plasma (heparin or EDTA)
Key=kit_components, value=10 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	51	227	971	59	238	984
Standard deviation	2.04	11.12	54.38	4.01	12.38	58.06
CV (%)	4.0%	4.9%	5.6%	6.8%	5.2%	5.9%

Key=application_details, value=Dilution ratio of 1:50, concentration in serum and plasma is around 20 ng/ml.
Key=subcellular_localization, value=Secreted.
Key=tissue_specificity, value=Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue.
Key=protein_function, value=Acts specifically as a negative regulator of skeletal muscle growth.
Key=sample_data, value=

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.076	0.150	0.185	0.292	0.520	0.839	1.496	2.092

Key=activating_reagent, value=Myostatin/GDF8 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant Myostatin/GDF8.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.
Key=sample_activation_procedure, value=

Activate the sample

Cell culture supernate: add activating reagent pro rata, i.e. add 50ul of Solution A into 100ul of sample, 10 min later, add 50ul of Solution B. PH 7.0-7.6.

Serum, plasma (heparin, EDTA): add activating reagent pro rata, i.e. add 20ul of Solution A into 40ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant Myostatin/GDF8.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: D268-S376
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	51	55	46	51	50	3.19	6.3%
Sample 2	227	216	255	226	231	14.50	6.2%
Sample 3	971	973	959	989	973	10.67	1.0%

*number of samples for each test n=16.
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