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Product Info Summary
| SKU: | EK1567 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Legumain(total) ELISA Kit PicoKine®
View all Legumain/Asparaginyl Endopeptidase ELISA kits
SKU/Catalog Number
EK1567
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Legumain (total) ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lgmn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Legumain(total) ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1567)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V18-Y435
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1567 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1567 is reactive to Lgmn in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1567 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Legumain/Asparaginyl Endopeptidase
Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1567-CAP | Anti-Mouse Lgmn Pre-coated 96-well strip microplate | 1 |
| EK1567-ST | Mouse Lgmn Standard | 2 vials, 10 ng/tube |
| EK1567-DA | Mouse Lgmn Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.157 | 0.207 | 0.222 | 0.308 | 0.522 | 0.921 | 1.448 | 2.465 |
Data Example Images
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Mouse Legumain(total) PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Legumain/Asparaginyl Endopeptidase, Dilution ratio of 1:100, concentration in serum and plasma is 16-50 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Legumain(total) ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 84 | 270 | 1001 | 90 | 264 | 954 |
| Standard deviation | 3.94 | 12.42 | 68.06 | 4.32 | 15.31 | 65.82 |
| CV (%) | 4.7% | 4.6% | 6.8% | 4.8% | 5.8% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Legumain/Asparaginyl Endopeptidase in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 84 | 80 | 93 | 92 | 87 | 5.44 | 6.2% |
| Sample 2 | 270 | 260 | 234 | 241 | 251 | 14.41 | 5.7% |
| Sample 3 | 1001 | 921 | 978 | 997 | 974 | 31.94 | 3.2% |
Protein Target Info & Infographic
Gene/Protein Information For Lgmn (Source: Uniprot.Org, NCBI)
Gene Name
Lgmn
Full Name
Legumain
Weight
49373 MW
Superfamily
peptidase C13 family
Alternative Names
Legumain;3.4.22.34;Asparaginyl endopeptidase;Protease, cysteine 1;Lgmn;Prsc1; LGMN AEP1, PRSC1, LGMN legumain legumain|asparaginyl endopeptidase|cysteine protease 1|protease, cysteine 1|protease, cysteine, 1 (legumain)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Lgmn, check out the Lgmn Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Lgmn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Legumain(total) ELISA Kit PicoKine® (EK1567)
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Customer Q&As
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8 Customer Q&As for Mouse Legumain(total) ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Mouse Legumain Picokine® ELISA Kit (Catalog # EK1567)?
Verified Customer
Verified customer
Asked: 2020-10-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Legumain causing degradation of its protein structure. Legumain may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Legumain ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
G. Miller
Verified customer
Asked: 2020-09-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-09-19
Question
Q: Can Legumain ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Garcia
Verified customer
Asked: 2020-06-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-06-16
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-23
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Legumain using the EK1567 Mouse Legumain Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-11-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Babu
Verified customer
Asked: 2019-09-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-22
Question
Q: how to thaw whole blood sample for Legumain ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Legumain for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-08-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
C. Mitchell
Verified customer
Asked: 2018-02-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-02-27
