Product Info Summary
Species: | Mouse |
Sample Type: | Serum, EDTA Plasma |
Volume Required per Well: | Min 25 µl |
Assay Length: | 2.25 hours |
Specificity: | All analytes < 1% intra-panel cross-reactivity |
Assay Type: | Planar Based Multiplexed ELISA |
Detection Method: | Chemiluminescent |
Multiplex Format: | 96-well solid plate |
Catalog: | MEK2003 |
Product info
Product Overview
Mouse Cytokine Chemi (4-plex) Sample Kit is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 4 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 4 markers in the panel within 2.25 hours. The Mouse Cytokine Chemi (4-plex) Sample Kit provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
*For a limited time, we are offering this kit on trial basis for only $99 per kit. Limit to 1 kit per lab. Claim yours today by contacting us at [email protected] for details.
Assay Ranges
Calibrator Range | Units | ||
IL-2 | 5000-6.86 | pg/ml | |
IL-4 | 5000-6.86 | pg/ml | |
IL-6 | 5000-6.86 | pg/ml | |
TNFα | 3000-14.12 | pg/ml |
Kit Components
Each kit contains a 96-well plate (not strip well), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
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